Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?

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dc.contributor.authorErgenoglu, Mete
dc.contributor.authorErbas, Oytun
dc.contributor.authorAkdemir, Ali
dc.contributor.authorYeniel, Ahmet Ozgur
dc.contributor.authorYildirim, Nuri
dc.contributor.authorOltulu, Fatih
dc.contributor.authorAktug, Huseyin
dc.contributor.authorTaskiran, Dilek
dc.date.accessioned2019-10-27T21:55:30Z
dc.date.available2019-10-27T21:55:30Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model. (C) 2013 S. Karger AG, Baselen_US
dc.identifier.doi10.1159/000353403en_US
dc.identifier.endpage32en_US
dc.identifier.issn0014-312X
dc.identifier.issn1421-9921
dc.identifier.issue01.Feben_US
dc.identifier.pmid23899611en_US
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.1159/000353403
dc.identifier.urihttps://hdl.handle.net/11454/48234
dc.identifier.volume51en_US
dc.identifier.wosWOS:000327768200003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKargeren_US
dc.relation.ispartofEuropean Surgical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPneumoperitoneumen_US
dc.subjectEdaravoneen_US
dc.subjectIschemia/reperfusion injuryen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectOvaryen_US
dc.titleAttenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?en_US
dc.typeArticleen_US

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