New Psychoactive Substance 5-MeO-MiPT in vivo Acute Toxicity and Hystotoxicological Study

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dc.contributor.authorAltunci, Yusuf Ali
dc.contributor.authorAydogdu, Melike
dc.contributor.authorAcikgoz, Eda
dc.contributor.authorGuven, Ummu
dc.contributor.authorDukagac, Fahriye
dc.contributor.authorAtasoy, Asli
dc.contributor.authorAkgur, Serap Annette
dc.date.accessioned2021-05-03T20:33:21Z
dc.date.available2021-05-03T20:33:21Z
dc.date.issued2021
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: The hallucinogenic tryptamine analog 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) causes social problems worldwide. There are several studies on the metabolism; however, not more studies were found in the literature on acute toxicity. Aims: To report the acute toxicity of 5-MeO-MiPT in mice, followed by quantitative toxicological analysis of blood and organs, hystotoxico-logical and immunohistochemical analysis of tissues and cells. Study design: Animal experiment Methods: in vivo experiments were performed using CD1 adult female mice (n=26). Animals were caged in 4 groups randomly. First group was a control (n=3). Second group was vehicle control (n=3) and injected 150 mu L of blank solution (50% dimethyl sulfoxide in saline /0.9% of NaCl). While for acute toxicity experiments, 5-MeO-MiPT was added to a blank solution in order to obtain a dose of 0.27 mg/kg in 150 mu L injection (n=10) and the last group were injected 2.7 mg/kg 5-MeO-MiPT in a 150 mu L injection (n=10). Quantitative toxicological analysis, hystotoxicological and immunohistochemical analysis were performed. Results: in the toxicological analysis, 5-MeO-MiPT was found negative in biological samples which were control, vehicle control, and 0.27 mg/kg dose mice groups. 5-MeO-MiPT was found 2.7-13.4 ng/mL in blood, 11-29 ng/g in kidney, 15.2-108.3 ng/g in liver, and 1.5-40.6 ng/g in the brain in 2,7 mg/kg injected group. in a low dose of the 5-MeO-MiPT liver section, compared with normal tissues, the difference in staining was statistically significant (p<0.0001). in high-dose of 5-MeO-MiPT, H-score showed that the increase in the number of Caspase-3 positive cells was significant compared to the control (p<0.05). in high-dose of 5-MeO-MiPT, intense Caspase-3 immunoreactivity was observed and the increase in the number of Caspase-3 positive cells compared to the control was statistically significant (p<0.05). in brain section, the statistics of the results obtained from the H-score showed that the increase in the number of Caspase-3 positive cells was significant compared to the control (p=0.0183). in vehicle control liver section, there were few Caspase-8 positive cells characterized by a light brown appearance (p=0.0117). in the high-dose 5-MeO-MiPT group, the numbers of positive cells at low and high doses of 5-MeO-MiPT group were statistically significant compared to the control (p<0.05). in the high-dose 5-MeO-MiPT group, Caspase-8 immunoreactivity was detected in the glomerular structures. Compared to control, the increase in Caspase-8 immunoreactivity was found to be statistically significant (p<0.05). Conclusion: Low-dose 5-MeO-MiPT did not cause any serious histopathological effects on the liver, kidney, and brain. High doses induce apoptotic cell death through caspase activity.en_US
dc.identifier.doi10.4274/balkanmedj.galenos.2020.2019.11.68en_US
dc.identifier.endpage42en_US
dc.identifier.issn2146-3123
dc.identifier.issn2146-3131
dc.identifier.issue1en_US
dc.identifier.pmid32936075en_US
dc.identifier.scopus2-s2.0-85102103193en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage34en_US
dc.identifier.urihttps://doi.org/10.4274/balkanmedj.galenos.2020.2019.11.68
dc.identifier.urihttps://hdl.handle.net/11454/69994
dc.identifier.volume38en_US
dc.identifier.wosWOS:000621685700006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofBalkan Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keyword]en_US
dc.titleNew Psychoactive Substance 5-MeO-MiPT in vivo Acute Toxicity and Hystotoxicological Studyen_US
dc.typeArticleen_US

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