New electroactive hybridization indicators 2-phthalimido-N-substitutedphenylethanesulfonamide derivatives for biosensor applications: Ring substituent effect on interaction between compound and DNA
Küçük Resim Yok
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this work, an electrochemical DNA-based sensor was developed for the detection of the interaction between the anticonvulsant compounds 2-phthalimido-N-substituted phenylethanesulfonamides (PMPES-derivatives) and 24-mer short DNA sequences by using differential pulse voltammetry (DPV) based on both compound and guanine oxidation signals at the renewable carbon graphite electrode (CGE) surface. The influence of compounds on DNA showed differences depending on the nature and position of the substituent on the N-phenyl ring. Compounds bearing 3-methoxy, 4-chloro and 2,6-dimethyl substituents bind to single stranded probe DNA more strongly than the other derivatives of PMPES. Thus, these compounds were evaluated for use as an electrochemical hybridization label (indicator). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Açıklama
Anahtar Kelimeler
2-Phthalimido-N-substitutedphenylethanesulfonamides, Biosensors, Carbon graphite electrode, DNA, Drug-DNA interactions, Guanine, Hybridization
Kaynak
Electroanalysis
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
22
Sayı
19
