Effects of passage number on proliferation and store-operated calcium entry in A7r5 vascular smooth muscle cells

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dc.contributor.authorErac, Yasemin
dc.contributor.authorSelli, Cigdem
dc.contributor.authorFilik, Pelin
dc.contributor.authorTosun, Metiner
dc.date.accessioned2019-10-27T22:14:02Z
dc.date.available2019-10-27T22:14:02Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractIntroduction: Embryonic rat aortic smooth muscle cells, A7r5, have been used extensively as an in vitro vascular smooth muscle cellmodel. They are usually provided at 11th passage by supplier and generally used before 25th passage. However, the exact passage number (P#) used is not reported in general. Methods: In this study, A7r5 cells (P# < 18 and P# > 23) were used in quantitative gene (5-HT2A and SERCA2b) expression as well as in functional analyses including measurements of real-time changes in cell proliferation and in 5-HT-and CPA-induced intracellular Ca2+ levels. Results: CPA-induced SR Ca2+ release and store-operated Ca2+ entry significantly increased (p < .05) while cell proliferation decreased in P# > 23 cells (p < .01). Furthermore, 5-HT-induced Ca2+ elevations and 5-HT2A mRNA levels did not change whereas SERCA2b mRNA levels and CPA-induced [ Ca2+](i) levels were significantly elevated in P# > 23 cells (p < .05, p < .01, respectively). Discussion: Changes in SERCA2b expression and SOCE may contribute to suppression of cell proliferation during A7r5 subculturing. Therefore, passage numbers and subculturing procedure should be reported and taken into account during expressional and functional analyses for an accurate comparison of published data. (C) 2014 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-3033]; Ege UniversityEge University [BAP09ECZ001]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, SBAG-3033 to MT) and in part by the Ege University (BAP09ECZ001 to MT).en_US
dc.identifier.doi10.1016/j.vascn.2014.03.001en_US
dc.identifier.endpage5en_US
dc.identifier.issn1056-8719
dc.identifier.issn1873-488X
dc.identifier.issue1en_US
dc.identifier.pmid24632210en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.vascn.2014.03.001
dc.identifier.urihttps://hdl.handle.net/11454/49929
dc.identifier.volume70en_US
dc.identifier.wosWOS:000341352500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal of Pharmacological and Toxicological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell cultureen_US
dc.subjectCell growthen_US
dc.subjectFura-2en_US
dc.subjectSubculturing methoden_US
dc.titleEffects of passage number on proliferation and store-operated calcium entry in A7r5 vascular smooth muscle cellsen_US
dc.typeArticleen_US

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