MerR-fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking water

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dc.contributor.authorOzyurt, Canan
dc.contributor.authorUstukarci, Handan
dc.contributor.authorEvran, Serap
dc.contributor.authorTelefoncu, Azmi
dc.date.accessioned2020-12-01T12:09:23Z
dc.date.available2020-12-01T12:09:23Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.descriptionEvran, Serap/0000-0001-6676-4888en_US
dc.description.abstractMercury ion (Hg2+) is a universal pollutant and its detection is crucial for public healthcare. in this study, we developed a novel fluorescent biosensor by construction of a protein fusion between the mercury-sensing transcription factor MerR and enhanced yellow fluorescent protein (EYFP). Hg2+-induced conformational change of MerR was transduced into fluorescence signal. Fluorescence intensity of the biosensor protein decreased with increasing concentrations of Hg2+ and a linear response was obtained in the range of 0.5-40 nM. the limit of detection was 0.5 nM, which was much lower than the maximum allowed level in water. the biosensor specificity was highly dependent on type and concentration of metal ion. the biosensor exhibited high specificity in a mixture of metal ions at 0.5 nM concentration. However, the interference effect was more pronounced at 40 nM concentration of metal ions. the measurement was completed in less than 1 Min with no need for sample preparation or preincubation steps. the biosensor achieved accurate and reliable detection in the spiked drinking water sample, as validated by the inductively coupled plasma optical emission spectrometry.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination UnitEge University [2011 FEN 035] Funding Source: Medlineen_US
dc.identifier.doi10.1002/bab.1805en_US
dc.identifier.endpage737en_US
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue5en_US
dc.identifier.pmid31411759en_US
dc.identifier.startpage731en_US
dc.identifier.urihttps://doi.org/10.1002/bab.1805
dc.identifier.urihttps://hdl.handle.net/11454/63433
dc.identifier.volume66en_US
dc.identifier.wosWOS:000491221600003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology and Applied Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHg2+en_US
dc.subjectfluorescent proteinen_US
dc.subjectmercury biosensoren_US
dc.titleMerR-fluorescent protein chimera biosensor for fast and sensitive detection of Hg2+ in drinking wateren_US
dc.typeArticleen_US

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