Immunoexpressions of embryonic and nonembryonic stem cell markers (Nanog, Thy-1, c-kit) and cellular connections (connexin 43 and occludin) on testicular tissue in thyrotoxicosis rat model

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dc.contributor.authorOltulu, F.
dc.contributor.authorAktug, H.
dc.contributor.authorUysal, A.
dc.contributor.authorTurgan, N.
dc.contributor.authorOktem, G.
dc.contributor.authorErbas, O.
dc.contributor.authorYavasoglu, N. U. Karabay
dc.contributor.authorYavasoglu, A.
dc.date.accessioned2019-10-27T20:24:48Z
dc.date.available2019-10-27T20:24:48Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, possible thyrotoxicosis-related histological changes in testicular tissues of rats with experimentally induced thyrotoxicosis model were evaluated on cellular connections and stem cell markers. Two experimental groups, thyrotoxicosis and control, each consisting of eight animals were used. Rats in the thyrotoxicosis group were injected intraperitoneally with 3,3,5-triiodo-l-thyronine (50 mu g/100 g body weight/day) for 10 days. At the end of the study, animals in both groups were anesthetized, and blood samples were collected for biochemical analyses. Their testes were dissected out and histological procedure was conducted to perform further histochemical, immunohistochemical analyses and tissue expression analysis by real-time polymerase chain reaction. Expression of the stem cell markers such as c-kit and Thy-1 significantly decreased in the testes of the thyrotoxicosis group compared with the control group; however, Nanog expression was not detected in any of the groups. Similarly, connexin 43 and occludin expressions were also found to be significantly lower in the thyrotoxicosis group. These results on cellular connections are supported with the tissue expression analysis. Our findings are indicative of supporting microenvironmental tissue decay rather than parenchyma damage, which has been actually ignored in the literature. In conclusion, experimental thyrotoxicosis model may have adverse effects on the cell junctional complexes, cell-cell interactions, and pluripotency capacity.en_US
dc.description.sponsorshipEge University, Faculty of MedicineEge University [2011/TIP/042]en_US
dc.description.sponsorshipThe study is supported by Ege University, Faculty of Medicine (project number is 2011/TIP/042).en_US
dc.identifier.doi10.1177/0960327114551392en_US
dc.identifier.endpage611en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue6en_US
dc.identifier.pmid25304966en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage601en_US
dc.identifier.urihttps://doi.org/10.1177/0960327114551392
dc.identifier.urihttps://hdl.handle.net/11454/42170
dc.identifier.volume34en_US
dc.identifier.wosWOS:000355334400004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThyrotoxicosisen_US
dc.subjecttestesen_US
dc.subjectconnexin 43en_US
dc.subjectoccludinen_US
dc.subjectNanogen_US
dc.subjectThy-1en_US
dc.subjectc-Kiten_US
dc.titleImmunoexpressions of embryonic and nonembryonic stem cell markers (Nanog, Thy-1, c-kit) and cellular connections (connexin 43 and occludin) on testicular tissue in thyrotoxicosis rat modelen_US
dc.typeArticleen_US

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