Genotyping of rifampin-resistant Mycobacterium tuberculosis isolates by mycobacterial interspersed repetitive unit - variable number tandem repeat (MIRU-VNTR) analysis

Basit Öğe Kaydı
dc.contributor.authorCavusoglu, Cengiz
dc.contributor.authorKaratas, Eylem
dc.contributor.authorSoeyler, Ilknur
dc.date.accessioned2019-10-27T19:33:46Z
dc.date.available2019-10-27T19:33:46Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractMolecular typing methods have greatly enhanced our understanding on epidemiology of tuberculosis and allowed us to identify outbreaks and intertransmission within populations. Recently, a set of 12 variable-number tandem repeat (VNTR), designated mycobacterial interspersed repetitive units (MIRU), has been described as being useful for the typing of M.tuberculosis. In this study, 26 rifampin (RIF) resistant M.tuberculosis isolates with known IS6110-RFLP patterns obtained from 26 different patients in Aegean Region were typed by MIRU-VNTR and the data were compared with IS6110-RFLP results. The results showed that in most isolates the clustering on the basis of IS6110 RFLP typing and that on the basis of MIRU-VNTR typing were in agreement. It was also determined that the loci including MIRU 16, MIRU 40, MIRU 26, MIRU 10, MIRU 04 and MIRU 31, respectively, have the highest allelic diversities and discriminatory power. In conclusion, since the discrimination level of conventional MIRU-VNTR including 12 loci might be variable, by the use of additional loci which present high degree of allelic differentiation, this method would be reliable for the epidemiologic studies.en_US
dc.identifier.endpage393en_US
dc.identifier.issn0374-9096
dc.identifier.issue3en_US
dc.identifier.pmid17933249en_US
dc.identifier.startpage385en_US
dc.identifier.urihttps://hdl.handle.net/11454/39525
dc.identifier.volume41en_US
dc.identifier.wosWOS:000249642000006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMIRU-VNTRen_US
dc.subjectIS6110-RFLPen_US
dc.subjectmolecular epidemiologyen_US
dc.subjectMycobacterium tuberculosisen_US
dc.titleGenotyping of rifampin-resistant Mycobacterium tuberculosis isolates by mycobacterial interspersed repetitive unit - variable number tandem repeat (MIRU-VNTR) analysisen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu