Radiolabeling of bombesin-like peptide with Tc-99m : Tc-99m-Litorin and biodistribution in rats

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dc.contributor.authorDurkan, Kubra
dc.contributor.authorLambrecht, Fatma Yurt
dc.contributor.authorUnak, Perihan
dc.date.accessioned2019-10-27T19:32:36Z
dc.date.available2019-10-27T19:32:36Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractBombesin-like peptides are related to several human cancer receptors, including small cell lung, prostate, breast, colon, and pancreatic cancers. Litorin, an amphibian bombesin peptide derivative, is found to stimulate the contraction of smooth muscle, to stimulate gastrin, gastric acid, and pancreatic secretion, and to suppress the nutriment in in vivo experiments. In the present study, litorin was labeled with 99mTc by the stannous chloride procedure. Labeling yield is 95 +/- 1.4%, as determined by radio thin layer chromatography (RTLC) and radio high performance chromatography (RHPLC). Results of in vitro studies demonstrated a high stability in serum and cysteine solutions. In vivo biodistribution was investigated with normal male Albino Wistar rats. Biodistribution data showed fast clearance, low intestinal accumulation, and significant uptake in bombesin/gastrin releasing peptide (BN/GRP) receptor rich tissues such as the pancreas (23.56 +/- 0.01 %ID/g 30 min pi). It can be blocked partially by previous administration of 'cold' litorin. The results showed specificity of the uptake. As Tc-99m-litorin displays good radiolabeling and biodistribution, it is a potentially useful radiopharmaceutical for detection of bombesin receptor-expressing cancers.en_US
dc.identifier.doi10.1021/bc060400xen_US
dc.identifier.endpage1520en_US
dc.identifier.issn1043-1802
dc.identifier.issue5en_US
dc.identifier.pmid17760415en_US
dc.identifier.startpage1516en_US
dc.identifier.urihttps://doi.org/10.1021/bc060400x
dc.identifier.urihttps://hdl.handle.net/11454/39312
dc.identifier.volume18en_US
dc.identifier.wosWOS:000249656100021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofBioconjugate Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleRadiolabeling of bombesin-like peptide with Tc-99m : Tc-99m-Litorin and biodistribution in ratsen_US
dc.typeArticleen_US

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