Genotyping ofPneumocystis jiroveciiisolates obtained from clinical samples by multilocus sequencing: a molecular epidemiology study conducted in Turkey

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dc.contributor.authorSurgec, Ecem
dc.contributor.authorCan, Huseyin
dc.contributor.authorDoskaya, Mert
dc.contributor.authorKarakavuk, Muhammet
dc.contributor.authorSahar, Esra Atalay
dc.contributor.authorDoskaya, Aysu Degirmenci
dc.contributor.authorDemir, Samiye
dc.date.accessioned2020-12-01T11:58:24Z
dc.date.available2020-12-01T11:58:24Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractPneumocystis jiroveciiis an opportunistic respiratory pathogen causing Pneumocystis pneumonia (PcP) in immunocompromised patients. the aim of this study was to investigate the genetic diversity ofP. jiroveciiisolates (n: 84) obtained from PcP patients using multilocus sequencing method based on mt26S, SOD, and CYB loci. Among the 84 clinical samples that were positive forP. jiroveciiDNA, 31 (36.90%) of them were genotyped using at least one locus. of the 31 clinical samples, 26 of them were successfully genotyped using all loci whereas three samples were genotyped using either mt26S/CYB loci or mt26S/SOD loci. Additionally, there were two more clinical samples that were genotyped using CYB or SOD locus. Using mt26S locus, genotypes 2, 3, 7, and 8 were detected. Frequencies of genotype 7 and 8 were higher and both of them were found in 11 (n: 29; 37.93%) clinical samples. Using SOD locus, SOD 1, 2, and 4 genotypes were detected. SOD 1 was the predominant genotype (20/28; 71.42%). During the analyses of CYB locus, CYB 1, 2, 5, 6, and 7 as well as a new CYB genotype were detected. CYB 1 (16/29; 55.17%) and 2 (10/29; 34.48%) were the predominant genotypes. Overall, according to the multilocus sequencing results E, F, M, N, P, and V multilocus genotypes were detected among the PcP patients. in addition, SOD 1 was the predominant genotype and CYB had a more polymorphic locus.en_US
dc.description.sponsorshipEge Universitesi [TGA-2019-20253] Funding Source: Medlineen_US
dc.identifier.doi10.1007/s00203-020-01874-3en_US
dc.identifier.endpage1652en_US
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue7en_US
dc.identifier.pmid32274557en_US
dc.identifier.scopus2-s2.0-85083059533en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1647en_US
dc.identifier.urihttps://doi.org/10.1007/s00203-020-01874-3
dc.identifier.urihttps://hdl.handle.net/11454/62014
dc.identifier.volume202en_US
dc.identifier.wosWOS:000552759000006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPneumocystis jiroveciien_US
dc.subjectCYBen_US
dc.subjectmt26Sen_US
dc.subjectSODen_US
dc.subjectGenotypingen_US
dc.titleGenotyping ofPneumocystis jiroveciiisolates obtained from clinical samples by multilocus sequencing: a molecular epidemiology study conducted in Turkeyen_US
dc.typeArticleen_US

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