Polypeptide Functional Surface for the Aptamer Immobilization: Electrochemical Cocaine Biosensing

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dc.contributor.authorBozokalfa, Guliz
dc.contributor.authorAkbulut, Huseyin
dc.contributor.authorDemir, Bilal
dc.contributor.authorGuler, Emine
dc.contributor.authorGumus, Z. Pinar
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorAldemir, Ebru
dc.contributor.authorYamada, Shuhei
dc.contributor.authorEndo, Takeshi
dc.contributor.authorCoskunol, Hakan
dc.contributor.authorTimur, Suna
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-10-27T23:10:20Z
dc.date.available2019-10-27T23:10:20Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractElectroanalytical technologies as a beneficial subject of modern analytical chemistry can play an important role for abused drug analysis which is crucial for both legal and social respects. This article reports a novel aptamer-based biosensing procedure for cocaine analysis by combining the advantages of aptamers as selective recognition elements with the well-known advantages of biosensor systems such as the possibility of miniaturization and automation, easy fabrication and modification, low cost, and sensitivity. In order to construct the aptasensor platform, first, polythiophene bearing polyalanine homopeptide side chains (PT-Pala) was electrochemically coated, onto the surface of an electrode and then cocaine aptamer was attached to the polymer via covalent conjugation chemistry. The stepwise modification of the surface was confirmed by electrochemical characterization. The designed biosensing system was applied for the detection of cocaine and its metabolite, benzoylecgonine (BE), which exhibited a linear correlation in the range from 2.5 up to 10 nM and 0.5 up to 50 mu M for cocaine and BE, respectively. In,order to expand its practical application, the proposed method was successfully tested for the analysis of synthetic biological fluids.en_US
dc.description.sponsorshipEge University Research FundEge University [15-Fen-020]en_US
dc.description.sponsorshipThis work was supported by Ege University Research Fund through the 15-Fen-020 project. B. Guler is acknowledged for the fruitful discussions.en_US
dc.identifier.doi10.1021/acs.analchem.6b00760en_US
dc.identifier.endpage4167en_US
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.issue7en_US
dc.identifier.pmid26928030en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4161en_US
dc.identifier.urihttps://doi.org/10.1021/acs.analchem.6b00760
dc.identifier.urihttps://hdl.handle.net/11454/52870
dc.identifier.volume88en_US
dc.identifier.wosWOS:000373656300098en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titlePolypeptide Functional Surface for the Aptamer Immobilization: Electrochemical Cocaine Biosensingen_US
dc.typeArticleen_US

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