Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®

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dc.contributor.authorInce I.
dc.contributor.authorKayalar H.
dc.contributor.authorElgin G.
dc.contributor.authorKoksal C.
dc.contributor.authorYavasoglu N.U.K.
dc.date.accessioned2019-10-26T21:49:02Z
dc.date.available2019-10-26T21:49:02Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractContext: Polyherbal formulations containing different plants are used for the treatment of various diseases. Romix® powder is a polyherbal formulation consisting of 14 traditionally used herbs and is used as a food supplement. There is no information about pharmaceutical activities of Romix®. Objective: This study determined the total phenolic and total flavonoid content, and investigated the antioxidant and anti-inflammatory activities and acute toxicity of Romix®. Material and methods: The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteu reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The quercetin content of the extract was analyzed using the high-performance liquid chromatography (HPLC) method. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the rat. Results: The flavonoid and phenolics contents of Romix® were 50.58 and 265.83mg/g in ethanol extract and 18.60 and 222.50mg/g in water extract, respectively. Total quercetin content of Romix® was determined as 2.857mg/g. Antioxidant activity results showed that ethanol extract in 1mg/mL concentration (4.49775 µg/mL) had moderate antioxidant activity than water extract in the same concentration (4.28191 µg/mL). Intraperitoneal administration of 25mg/kg Romix® extract exhibited anti-inflammatory activity and inhibited paw swelling at 1, 2, 3, 4, 5 and 6h in rats with no acute toxicity. Conclusion: These findings suggest that Romix® due to its antioxidant and anti-inflammatory activities may be useful in the prevention or treatment of aging-related and inflammatory diseases. © 2012 Informa Healthcare USA, Inc.en_US
dc.description.sponsorshipThis work was supported by the Naturin Natural Products, Drug and Drug Raw Material Ltd. Izmir, Turkey. --en_US
dc.identifier.doi10.3109/13880209.2011.622287en_US
dc.identifier.endpage726en_US
dc.identifier.issn1388-0209
dc.identifier.issue6en_US
dc.identifier.pmid22571398en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage720en_US
dc.identifier.urihttps://doi.org/10.3109/13880209.2011.622287
dc.identifier.urihttps://hdl.handle.net/11454/18686
dc.identifier.volume50en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofPharmaceutical Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFlavonoid contenten_US
dc.subjectHPLCen_US
dc.subjectPhenolic contenten_US
dc.subjectQuercetin contenten_US
dc.titleAntioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®en_US
dc.typeArticleen_US

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