Determination of cut-off titers and agreement between immunofluorescence and immunoblotting methods for detecting antinuclear antibodies in children

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dc.contributor.authorAksu G.
dc.contributor.authorGulez N.
dc.contributor.authorAzarsiz E.
dc.contributor.authorKaraca N.
dc.contributor.authorKutukçuler N.
dc.date.accessioned2019-10-26T22:32:44Z
dc.date.available2019-10-26T22:32:44Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractDetection of antinuclear antibodies (ANA) is a diagnostic adjunct in patients with suspected autoimmune connective tissue diseases, and various detection methods are in use. The aim of this study was to analyze the agreement between the ANA immunoflourescence (IF) and immunoblotting (IB) methods and determine cut-off for children subjects in a laboratory setting. We evaluated 729 serum samples that were analyzed by both ANA IF and IB. The results were evaluated by ? 2 test and, for agreement, ? index was used. Frequencies determined for both 1:40-1:100 cut-off titers of ANA IF in relation to IB testing supported the idea that 1:100 starting dilution should be recommended in children subjects for ANA IF method and antigen specific immunoblot testing was needed, especially for some of the ANA IF negative samples. Agreement between the two methods, especially with homogenous, granular, and nucleolar ANA IF patterns, was statistically significant. © 2010 Wiley-Liss, Inc.en_US
dc.identifier.doi10.1002/jcla.20391en_US
dc.identifier.endpage236en_US
dc.identifier.issn0887-8013
dc.identifier.issue4en_US
dc.identifier.pmid20626019en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage230en_US
dc.identifier.urihttps://doi.org/10.1002/jcla.20391
dc.identifier.urihttps://hdl.handle.net/11454/19761
dc.identifier.volume24en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Clinical Laboratory Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectANAen_US
dc.subjectChildrenen_US
dc.subjectCut-off titeren_US
dc.subjectImmunoblottingen_US
dc.subjectImmunoflourescenceen_US
dc.titleDetermination of cut-off titers and agreement between immunofluorescence and immunoblotting methods for detecting antinuclear antibodies in childrenen_US
dc.typeArticleen_US

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