Comparison of 12 liquid media for germ tube production of Candida albicans and C. tropicalis

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dc.contributor.authorHilmioglu S.
dc.contributor.authorIlkit M.
dc.contributor.authorBadak Z.
dc.date.accessioned2019-10-26T23:59:28Z
dc.date.available2019-10-26T23:59:28Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractInfections caused by yeast of the genus Candida are the most common fungal infections, being Candida albicans the most common isolated species among them. The rapid identification of this yeast is mostly based on the production of germ tube in human or animal serum. This study describes the use of 12 different liquid media for germ tube production at 2, 2.5, 3 and 4 h. We examined 193 yeasts, including 157 (81.3%) C. albicans and 36 (18.7%) Candida tropicalis for the production of germ tube. The germ tube production of C. albicans was mostly observed in human serum (98%) followed by rabbit serum (89.8%), brain heart infusion broth (84%) and sheep serum (74.5%) at 2 h. An incubation time exceeding 2 h i.e. 2.5 h or later, C. tropicalis strains were observed to produce germ tubes. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for germ tube production of human serum at 2 h were 98%, 100%, 100% and 92.3% respectively. In all tested sera, an incubation period of more than 2 h improves the sensitivity, but decreases the specificity as well as PPV and NPV of germ tube test (GTT). In conclusion, human serum was observed to be the most appropriate medium to be preferred for GTT, with an incubation period of 2 h. © 2007 The Authors.en_US
dc.identifier.doi10.1111/j.1439-0507.2007.01359.xen_US
dc.identifier.endpage285en_US
dc.identifier.issn0933-7407
dc.identifier.issue4en_US
dc.identifier.pmid17576320en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage282en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0507.2007.01359.x
dc.identifier.urihttps://hdl.handle.net/11454/21399
dc.identifier.volume50en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofMycosesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCandida albicansen_US
dc.subjectCandida tropicalisen_US
dc.subjectGerm tubeen_US
dc.titleComparison of 12 liquid media for germ tube production of Candida albicans and C. tropicalisen_US
dc.typeArticleen_US

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