Optical detection based spot test on electrospun nanofibers for glioblastoma cells

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dc.authorid0000-0002-3868-0801
dc.authorid0000-0002-3808-724X
dc.authorid0000-0001-7028-0720
dc.authorid0000-0003-0139-7110
dc.authorid0000-0002-7954-1381
dc.contributor.authorUs, Aybuke Elif
dc.contributor.authorKirbay, Fatma Ozturk
dc.contributor.authorGuldu, Ozge Kozgus
dc.contributor.authorOdaci, Dilek
dc.date.accessioned2025-02-24T13:03:53Z
dc.date.available2025-02-24T13:03:53Z
dc.date.issued2024
dc.departmentEge Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
dc.description.abstractPaper-based diagnosis is enabled to be used in many applications in global health due to its low cost and simplicity of operation. Electrospun nanofibers (ESNFs) are useful platforms to prepare paper-based diagnostics. Herein, bead-free ESNFs were formed by electrospinning using polystyrene (PS) and poly (ethylene glycol) (PEG) polymers as a paper-based substrate. PS:PEG ESNFs were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), identification of swelling behavior, and Brunauer-Emmett-Teller (BET) analysis. Synthesized gold nanoparticles (AuNPs) were used as a colorimetric probe and GMT8 aptamer was conjugated on the surface of AuNPs. AuNP and AuNP-GMT8 were characterized by dynamic light scattering (DLS), UV-Visible spectrometry, and X-Ray photoelectron spectroscopy (XPS). The interaction of AuNP-GMT8 bioconjugates with glioblastoma cells was examined. The colored spots for the mixture of glioblastoma (U87MG) cells and AuNP-GMT8 were optically monitored both in the microplate wells and on the PS:PEG ESNFs. The linear range of colorimetric analysis on PS:PEG ESNFs was determined to be between 101-106 U87-MG cells/mL by smartphone using RGB color analysis.
dc.identifier.citationUs, A. E., Kirbay, F. O., Guldu, O. K., Medine, E. I., & Odaci, D. (2025). Optical detection based spot test on electrospun nanofibers for glioblastoma cells. Talanta (Oxford), 285, 127303.
dc.identifier.doi10.1016/j.talanta.2024.127303
dc.identifier.endpage10
dc.identifier.issn0039-9140
dc.identifier.issueDec
dc.identifier.pmid39637774
dc.identifier.scopus2-s2.0-85210637104
dc.identifier.scopusqualityQ1
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2024.127303
dc.identifier.urihttps://hdl.handle.net/11454/116190
dc.identifier.volume285
dc.identifier.wosWOS:001375716900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorUs, Aybuke Elif
dc.institutionauthorKirbay, Fatma Ozturk
dc.institutionauthorGuldu, Ozge Kozgus
dc.institutionauthorMedine, Emin Ilker
dc.institutionauthorOdaci, Dilek
dc.institutionauthorid0000-0002-3868-0801
dc.institutionauthorid0000-0002-3808-724X
dc.institutionauthorid0000-0001-7028-0720
dc.institutionauthorid0000-0003-0139-7110
dc.institutionauthorid0000-0002-7954-1381
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAptamer
dc.subjectGlioblastoma
dc.subjectNanofiber
dc.subjectNanomaterial
dc.subjectNanotechnology
dc.titleOptical detection based spot test on electrospun nanofibers for glioblastoma cells
dc.typeArticle

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