Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages

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dc.contributor.authorEkizceli, Gulcin
dc.contributor.authorInan, Sevinc
dc.contributor.authorOktem, Gulperi
dc.contributor.authorOnur, Ece
dc.contributor.authorOzbilgin, Kemal
dc.date.accessioned2021-05-03T20:36:53Z
dc.date.available2021-05-03T20:36:53Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractmTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/ mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process.en_US
dc.description.sponsorshipCelal Bayar University, Scientific Research Project CommitteeCelal Bayar University [2011/038]en_US
dc.description.sponsorshipThis research was granted by Celal Bayar University, Scientific Research Project Committee. Number 2011/038.en_US
dc.identifier.doi10.14670/HH-18-258en_US
dc.identifier.endpage1351en_US
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.issue11en_US
dc.identifier.pmid32940340en_US
dc.identifier.scopus2-s2.0-85099592203en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1337en_US
dc.identifier.urihttps://doi.org/10.14670/HH-18-258
dc.identifier.urihttps://hdl.handle.net/11454/70262
dc.identifier.volume35en_US
dc.identifier.wosWOS:000608634300011en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherF Hernandezen_US
dc.relation.ispartofHistology and Histopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectERKen_US
dc.subjectEstrous cycleen_US
dc.subjectIGF1en_US
dc.subjectmTORC1en_US
dc.subjectOvulationen_US
dc.subjectpAKTen_US
dc.titleImmunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stagesen_US
dc.typeArticleen_US

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