In vivo radioprotective effects of Nigella sativa L oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats

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dc.contributor.authorCemek, Mustafa
dc.contributor.authorEnginar, Huseyin
dc.contributor.authorKaraca, Turan
dc.contributor.authorUnak, Perihan
dc.date.accessioned2019-10-27T19:40:43Z
dc.date.available2019-10-27T19:40:43Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractRadiotherapy is one of the most common therapies for treating human cancers. Several studies have indicated that irradiation induces reactive oxygen species (ROS), which play an important role in radiation damage of the cell. It has been shown that Nigella sativa L. (NS) and reduced glutathione (GSH) have both an antiperoxidative effect on different tissues and a scavenger effect on ROS. The purpose of this study was to determine the antioxidant and radioprotective roles of INS and GSH against irradiation-induced oxidative injury in an experimental model. The INS group was administrated INS (1 mL/kg body weight), the GSH group was injected GSH (150 mg/kg body weight) and the control group was given physiologic saline solution (1 mL/kg body weight) for 30 consecutive days before exposure to a single dose of 6 Gy of radiation. Animals were sacrificed after irradiation. Malondialdehyde, nitrate, nitrite (oxidative stress markers) and ascorbic acid, retinol, beta-carotene, GSH and ceruloplasmin (nonenzymatic antioxidant markers) levels and peripheral blood lymphocytes were measured in all groups. There were statistically significant differences between the groups for all parameters (P < 0.05). Whole-body irradiation caused a significant increase in blood malondial-dehyde, nitrate and nitrite levels. The blood oxidative stress marker levels in irradiated rats that were pretreated with NS and GSH were significantly decreased; however, nonenzymatic antioxidant levels were significantly increased. Also, our results suggest that NS and GSH administration prior to irradiation prevent the number of alpha-naphthyl acetate esterase peripheral blood T lymphocytes from declining. These results clearly show that NS and GSH treatment significantly antagonize the effects of radiation. Therefore, NS and GSH may be a beneficial agent in protection against ionizing radiation-related tissue injury.en_US
dc.identifier.doi10.1562/2006-06-15-RA-924en_US
dc.identifier.endpage1696en_US
dc.identifier.issn0031-8655
dc.identifier.issn1751-1097
dc.identifier.issue6en_US
dc.identifier.pmid17387769en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1691en_US
dc.identifier.urihttps://doi.org/10.1562/2006-06-15-RA-924
dc.identifier.urihttps://hdl.handle.net/11454/40286
dc.identifier.volume82en_US
dc.identifier.wosWOS:000243214100044en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofPhotochemistry and Photobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleIn vivo radioprotective effects of Nigella sativa L oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in ratsen_US
dc.typeArticleen_US

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