Genotypic discrimination of Aspergillus fumigatus strain from related species within section fumigati

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dc.contributor.authorGiray, Betul
dc.contributor.authorKocaogut, Elif
dc.contributor.authorUcar, Fusun B.
dc.contributor.authorHaliki-Uztan, Alev
dc.date.accessioned2019-10-27T22:57:54Z
dc.date.available2019-10-27T22:57:54Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractIntroduction and objective. The aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the Oncology and other departments of the Ege University Medical Faculty Hospital, and identified as Aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns. Materials and method. It was confirmed that the 20 phenotypically-identified A. fumigatus strains belonged to the section Fumigati after they were tested by the ITS-PCR method. Their sequence analysis was performed and the results sent to the NCBI GenBank, and their accession numbers were obtained. For their exact diagnosis at the species level, the beta-tub (beta-tubulin) and rodA (RodletA) genes were examined with the multiplex PCR. Anti-fungal susceptibility of the 20 strains was determined according to the M38-A2 standards. Results. As a result of ITS-PCR, it was confirmed that 19 of the 20 strains identified as A. fumigatus through the phenotypic methods belonged to the section Fumigati. However, after the detection of beta-tub and rodA genes, all 20 strains were identified as A. fumigatus. Conclusion. Although the results of the phenotypic and molecular biological tests applied to filamentous fungi do not often overlap, in this study, the results obtained from the molecular analysis confirmed the results of the phenotypic tests. However, 1 of the 20 strains phenotypically-identified as A. fumigatus was identified as Penicillium spp. as a result of ITS-PCR and sequence analysis. On the other hand, the profile obtained from beta-tub and rodA tests indicated that the strain was A. fumigatus. Based on these results, this strain is thought to belong to the Aspergilloides genus which has the features of both genera.en_US
dc.description.sponsorshipEU Scientific Research Project (BAP) [2013-FEN-14]en_US
dc.description.sponsorshipThe authors thank Assoc. Prof. Dr. Ozlem Abaci-Gunyar, PhD student Aysegul Yoltas, and Master's Degree student Erhan Sakartepe for isolation and phenotypical identification of the 20 A. fumigatus strains. Thanks are due also to Assoc. Prof. Dr. Dilek Yesim Metin for the preparation of the antifungal drugs. The study was supported by the EU Scientific Research Project (BAP) 2013-FEN-14.en_US
dc.identifier.doi10.5604/12321966.1219185en_US
dc.identifier.endpage451en_US
dc.identifier.issn1232-1966
dc.identifier.issn1898-2263
dc.identifier.issue3en_US
dc.identifier.pmid27660866en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage448en_US
dc.identifier.urihttps://doi.org/10.5604/12321966.1219185
dc.identifier.urihttps://hdl.handle.net/11454/51369
dc.identifier.volume23en_US
dc.identifier.wosWOS:000385026400011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInst Agricultural Medicineen_US
dc.relation.ispartofAnnals of Agricultural and Environmental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectrodAen_US
dc.subjectbeta tuben_US
dc.subjectA. fumigatusen_US
dc.subjectITS-PCRen_US
dc.subjectantifungal susceptibility testingen_US
dc.titleGenotypic discrimination of Aspergillus fumigatus strain from related species within section fumigatien_US
dc.typeArticleen_US

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