Comprehensive Analysis of Lutein and Loroxanthin in Scenedesmus obliquus: From Quantification to Isolation

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dc.authoridErdoğan, Ayşegül/0000-0002-3174-7970
dc.authoridKarataş, Ayça Büşra/0000-0001-7039-5579
dc.authoridDemirel, Zeliha/0000-0003-3675-7315
dc.contributor.authorErdogan, Aysegul
dc.contributor.authorKaratas, Ayca Busra
dc.contributor.authorDemir, Dilan
dc.contributor.authorDemirel, Zeliha
dc.contributor.authorAkturk, Merve
dc.contributor.authorCopur, Oyku
dc.contributor.authorCagir, Ali
dc.date.accessioned2024-08-31T07:50:18Z
dc.date.available2024-08-31T07:50:18Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractCarotenoids are hydrophobic pigments produced exclusively by plants, fungi, and specific microbes. Microalgae are well suited for the production of valuable carotenoids due to their rapid growth, efficient isoprenoid production pathway, and ability to store these compounds within their cells. The possible markets for bio-products range from feed additives in aquaculture and agriculture to pharmaceutical uses. The production of carotenoids in microalgae is affected by several environmental conditions, which can be utilized to enhance productivity. The current study focused on optimizing the extraction parameters (time, temperature, and extraction number) to maximize the yield of carotenoids. Additionally, the impact of various nitrogen sources (ammonia, nitrate, nitrite, and urea) on the production of lutein and loroxanthin in Scenedesmus obliquus was examined. To isolate the carotenoids, 0.20 g of biomass was added to 0.20 g of CaCO3 and 10.0 mL of ethanol solution containing 0.01% (w/v) pyrogallol. Subsequently, the extraction was performed using an ultrasonic bath for a duration of 10 min at a temperature of 30 degrees C. This was followed by a four-hour saponification process using a 10% methanolic KOH solution. The concentration of lutein and loroxanthin was measured using HPLC-DAD at 446 nm, with a flow rate of 1.0 mL/min using a Waters YMC C30 Carotenoid column (4.6 x 250 mm, 5 mu m). The confirmation of carotenoids after their isolation using preparative chromatography was achieved using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an atmospheric pressure chemical ionization (APCI) probe and UV-vis spectroscopy. In summary, S. obliquus shows significant promise for the large-scale extraction of lutein and loroxanthin. The findings of this study provide strong support for the application of this technology to other species.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) through the COST Project [216Z167]en_US
dc.description.sponsorshipThis research was funded by the Scientific and Technological Research Council of Turkiye (TUBITAK) through the COST Project 216Z167.en_US
dc.identifier.doi10.3390/molecules29061228
dc.identifier.issn1420-3049
dc.identifier.issue6en_US
dc.identifier.pmid38542865en_US
dc.identifier.scopus2-s2.0-85189180738en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/molecules29061228
dc.identifier.urihttps://hdl.handle.net/11454/105187
dc.identifier.volume29en_US
dc.identifier.wosWOS:001193575500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectScenedesmus Obliquusen_US
dc.subjectCarotenogenesisen_US
dc.subjectExtractionen_US
dc.subjectLuteinen_US
dc.subjectLoroxanthinen_US
dc.subjectNitrogen Sourcesen_US
dc.titleComprehensive Analysis of Lutein and Loroxanthin in Scenedesmus obliquus: From Quantification to Isolationen_US
dc.typeArticleen_US

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