Endoplasmic reticulum stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of Arabidopsis thaliana

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dc.contributor.authorOzgur, Rengin
dc.contributor.authorTurkan, Ismail
dc.contributor.authorUzilday, Baris
dc.contributor.authorSekmen, Askim H.
dc.date.accessioned2019-10-27T22:05:19Z
dc.date.available2019-10-27T22:05:19Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractEndoplasmic reticulum stress, which is induced by tunicamycin, triggers reactive oxygen species signalling via NADPH oxidase activity and also regulates the antioxidant defence system in Arabidopsis thaliana.Inefficient chaperone activity in endoplasmic reticulum (ER) causes accumulation of unfolded proteins and is called ER stress, which triggers the unfolded protein response. For proper oxidative protein folding, reactive oxygen species (ROS) such as H2O2 are produced in the ER. Although the role of ROS during abiotic stresses such as salinity is well documented, the role of ER-related ROS production and its signalling is not yet known. Moreover, how H2O2 production, redox regulation, and antioxidant defence are affected in salt-treated plants when ER protein-folding machinery is impaired needs to be elucidated. For this aim, changes in NADPH-oxidase-dependent ROS signalling and H2O2 content at sequential time intervals and after 48h of ER stress, induced by tunicamycin (Tm), salinity, and their combination were determined in Arabidopsis thaliana. The main root growth was inhibited by ER stress, while low levels of Tm caused an increase in lateral root density. Salt stress and Tm induced the expression of ER-stress-related genes (bZIP17, bZIP28, bZIP60, TIN1, BiP1, BiP3) and ERO1. Tm induced expression of RBOHD and RBOHF, which led to an early increase in H2O2 and triggered ROS signalling. This study is the first report that ER stress induces the antioxidant system and the AsadaHalliwell pathway of A. thaliana in a similar way to salinity. ER stress caused oxidative damage, as evident by increased H2O2 accumulation, lipid peroxidation, and protein oxidation. As a result, this study shows that ER stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of A. thaliana.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [212T018]; Ege University Research FoundationEge University [2013/BIL/016]en_US
dc.description.sponsorshipThe authors would like to thank Assoc. Prof. Dr. H. Caglar Karakaya from Izmir Institute of Technology for his valuable technical assistance. This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant no. 212T018) and the Ege University Research Foundation (grant no. 2013/BIL/016).en_US
dc.identifier.doi10.1093/jxb/eru034en_US
dc.identifier.endpage1390en_US
dc.identifier.issn0022-0957
dc.identifier.issn1460-2431
dc.identifier.issue5en_US
dc.identifier.pmid24558072en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1377en_US
dc.identifier.urihttps://doi.org/10.1093/jxb/eru034
dc.identifier.urihttps://hdl.handle.net/11454/48341
dc.identifier.volume65en_US
dc.identifier.wosWOS:000334099900012en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofJournal of Experimental Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntioxidant defenceen_US
dc.subjectendoplasmic reticulum stressen_US
dc.subjectROS signallingen_US
dc.subjectoxidative stressen_US
dc.subjectsalt stressen_US
dc.subjecttunicamycinen_US
dc.titleEndoplasmic reticulum stress triggers ROS signalling, changes the redox state, and regulates the antioxidant defence of Arabidopsis thalianaen_US
dc.typeArticleen_US

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