HN1 interacts with gamma-tubulin to regulate centrosomes in advanced prostate cancer cells

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dc.authoridJaved, Aadil/0000-0001-9098-2340
dc.authoridGAGOS, SARANTIS/0000-0003-4907-7706
dc.authoridKorkmaz, Kemal Sami/0000-0003-4040-2568
dc.authoridTakir, Gizem Gulevin/0000-0003-0724-2136
dc.authoridVarisli, Lokman/0000-0002-4337-9327
dc.authorscopusid16687246300
dc.authorscopusid57617592200
dc.authorscopusid57074478000
dc.authorscopusid57074559100
dc.authorscopusid57226665464
dc.authorscopusid56295290900
dc.authorscopusid6602669333
dc.authorwosidJaved, Aadil/AAP-3326-2021
dc.authorwosidVarisli, Lokman/GLR-5913-2022
dc.contributor.authorVarisli, Lokman
dc.contributor.authorJaved, Aadil
dc.contributor.authorOzturk, Bilge Esin
dc.contributor.authorAkyuz, Gencer Kaan
dc.contributor.authorTakir, Gulevin
dc.contributor.authorRoumelioti, Fani-Marlen
dc.contributor.authorGagos, Sarantis
dc.date.accessioned2023-01-12T19:59:56Z
dc.date.available2023-01-12T19:59:56Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractProstate cancer is one of the most common cancer for men worldwide with advanced forms showing supernumerary or clustered centrosomes. Hematological and neurological expressed 1 (HN1) also known as Jupiter Microtubule Associated Homolog 1 (JPT1) belongs to a small poorly understood family of genes that are evolutionarily conserved across vertebrate species. The co-expression network of HN1 from the TCGA PRAD dataset indicates the putative role of HN1 in centrosome-related processes in the context of prostate cancer. HN1 expression is low in normal RWPE-1 cells as compared to cancerous androgen-responsive LNCaP and androgen insensitive PC-3 cells. HN1 overexpression resulted in differential response for cell proliferation and cell cycle changes in RWPE-1, LNCaP, and PC-3 cells. Since HN1 overexpression increased the proliferation rate in PC-3 cells, these cells were used for functional characterization of HN1 in advanced prostate carcinogenesis. Furthermore, alterations in HN expression led to an increase in abnormal to normal nuclei ratio and increased chromosomal aberrations in PC-3 cells. We observed the co-localization of HN1 with gamma-tubulin foci in prostate cancer cells, further validated by immunoprecipitation. HN1 was observed as physically associated with gamma-tubulin and its depletion led to increased gamma-tubulin foci and disruption in microtubule spindle assembly. Higher HN1 expression was correlated with prostate cancer as compared to normal tissues. The restoration of HN1 expression after silencing suggested that it has a role in centrosome clustering, implicating a potential role of HN1 in cell division as well as in prostate carcinogenesis warranting further studies.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [113Z083]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey [113Z083].en_US
dc.identifier.doi10.1080/15384101.2021.1962624
dc.identifier.endpage1744en_US
dc.identifier.issn1538-4101
dc.identifier.issn1551-4005
dc.identifier.issue17en_US
dc.identifier.pmid34382911en_US
dc.identifier.scopus2-s2.0-85112328215en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1723en_US
dc.identifier.urihttps://doi.org/10.1080/15384101.2021.1962624
dc.identifier.urihttps://hdl.handle.net/11454/77260
dc.identifier.volume20en_US
dc.identifier.wosWOS:000684439100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofCell Cycleen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectProstate Canceren_US
dc.subjectHN1en_US
dc.subjectCentrosomeen_US
dc.subjectChromosomeen_US
dc.subjectSpindle Assemblyen_US
dc.subjectCycle Regulationen_US
dc.subjectOvarian-Canceren_US
dc.subjectDna-Synthesisen_US
dc.subjectIn-Vivoen_US
dc.subjectAmplificationen_US
dc.subjectGeneen_US
dc.subjectLocalizationen_US
dc.subjectInstabilityen_US
dc.subjectExpressionen_US
dc.subjectBindingen_US
dc.titleHN1 interacts with gamma-tubulin to regulate centrosomes in advanced prostate cancer cellsen_US
dc.typeArticleen_US

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