Molecular characterization of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) gene and frequency of blood types in stray cats of İzmir, Turkey

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dc.contributor.authorCan H.
dc.contributor.authorErkunt Alak S.
dc.contributor.authorKöseoğlu A.E.
dc.contributor.authorŞahar U.
dc.contributor.authorBostanbaş B.
dc.contributor.authorBaydarlı S.
dc.contributor.authorÜn C.
dc.date.accessioned2021-05-03T20:56:49Z
dc.date.available2021-05-03T20:56:49Z
dc.date.issued2021
dc.description.abstractBackground: Cytidine monophospho-n-acetylneuraminic acid hydroxylase (CMAH) gene associated with blood groups in cats encodes CMAH enzyme that converts Neu5Ac to Neu5Gc. Although variations in CMAH gene of pedigree cats have been revealed, the presence/lack of them in non-pedigree stray cats is unknown. Therefore, the present study aimed to investigate the variations in CMAH gene and the quantity of Neu5Ac and Neu5Gc on erythrocytes of non-pedigree stray cats (n:12) living in İzmir, Turkey. Also, the frequency of blood types was determined in 76 stray cats including 12 cats that were used for CMAH and Neu5A/Neu5Gc analysis. Results: In total, 14 SNPs were detected in 5’UTR as well as in exon 2, 4, 9, 10, 11 and 12 of CMAH gene. Among these SNPs, -495 C > T in 5’UTR was detected for the first time as heterozygous in type A and AB cats, and homozygous and heterozygous in type B cats. The remaining 13 that have been detected in previous studies were also found as homozygous or heterozygous. Both Neu5Gc and Neu5Ac were detected in type A and AB cats. In type B cats, only Neu5Ac was detected. Among two type AB cats, the level of Neu5Ac was found higher in cat carrying heterozygous form (T/C) of 1392T > C. The prevalence of type B cats (67.1 %) was higher than others. Conclusions: The presence of a new SNP as well as previous SNPs indicates that more variations can be found in stray cats with a more comprehensive study in the future. Also, the high prevalence of type B cats demonstrates the possible risk of neonatal isoerythrolysis among stray cats living in İzmir, Turkey. © 2021, The Author(s).en_US
dc.description.sponsorship218Z105 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipThis study was supported by a project given by The Scientific and Technological Research Council of Turkey (TÜBİTAK) (Project number: 218Z105) to C.Ü. The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.en_US
dc.identifier.doi10.1186/s12864-021-07588-0en_US
dc.identifier.issn1471-2164
dc.identifier.issue1en_US
dc.identifier.pmid33874895en_US
dc.identifier.scopus2-s2.0-85104670002en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1186/s12864-021-07588-0
dc.identifier.urihttps://hdl.handle.net/11454/71381
dc.identifier.volume22en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBioMed Central Ltden_US
dc.relation.ispartofBMC Genomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBlood typingen_US
dc.subjectCMAH geneen_US
dc.subjectStray catsen_US
dc.subjectTurkeyen_US
dc.titleMolecular characterization of cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) gene and frequency of blood types in stray cats of İzmir, Turkeyen_US
dc.typeArticleen_US

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