Stabilization of papain by modification with chitosan

dc.contributor.authorAli Kılınç
dc.contributor.authorSeçil Önal
dc.contributor.authorAzmi Telefoncu
dc.date.accessioned2019-10-26T19:54:14Z
dc.date.available2019-10-26T19:54:14Z
dc.date.issued2002
dc.departmentEge Üniversitesien_US
dc.description.abstractPapain (EC 3.4.22.2) was immobilized on chitosan by adsorption and subsequent cross-linking with glutaraldehyde. The immobilized papain displayed a lower specific activity than did the native enzyme. The thermal stability of the immobilized papain, relative to that of the free enzyme, was markedly increased. The storage stability of the conjugated enzyme was enhanced such that more than 85% of the initial activity remained after a month storage at 45°C. The optimum pH of immobilized papain was shifted to the acidic region and enzyme stability was increased at pH levels below 3.5.en_US
dc.description.abstractPapain (EC 3.4.22.2) was immobilized on chitosan by adsorption and subsequent cross-linking with glutaraldehyde. The immobilized papain displayed a lower specific activity than did the native enzyme. The thermal stability of the immobilized papain, relative to that of the free enzyme, was markedly increased. The storage stability of the conjugated enzyme was enhanced such that more than 85% of the initial activity remained after a month storage at 45°C. The optimum pH of immobilized papain was shifted to the acidic region and enzyme stability was increased at pH levels below 3.5.en_US
dc.identifier.endpage316en_US
dc.identifier.issn1300-0527
dc.identifier.issue3en_US
dc.identifier.startpage311en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpJME5qWTI=
dc.identifier.urihttps://hdl.handle.net/11454/14089
dc.identifier.volume26en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMühendisliken_US
dc.subjectKimyaen_US
dc.titleStabilization of papain by modification with chitosanen_US
dc.typeArticleen_US

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