Rapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Source

Basit Öğe Kaydı
dc.authoridAtalay, Necati/0000-0002-5372-9896
dc.authoridCetinok, Haluk/0000-0003-4301-4683
dc.authoridSHAFIEI, ALALEH/0000-0003-2424-9583
dc.authorscopusid57219160859
dc.authorscopusid57222395454
dc.authorscopusid57222396595
dc.authorscopusid57383249900
dc.authorscopusid57222396113
dc.authorscopusid55884290600
dc.authorscopusid57954068900
dc.authorwosidAtalay, Necati/HTL-9515-2023
dc.contributor.authorGul, Mehmet
dc.contributor.authorAyan, Esra
dc.contributor.authorDestan, Ebru
dc.contributor.authorJohnson, J. Austin
dc.contributor.authorShafiei, Alaleh
dc.contributor.authorKepceoglu, Abdullah
dc.contributor.authorYilmaz, Merve
dc.date.accessioned2024-08-25T18:48:11Z
dc.date.available2024-08-25T18:48:11Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractHigh-resolution biomacromolecular structure determination is essential to better understand protein function and dynamics. Serial crystallography is an emerging structural biology technique which has fundamental limitations due to either sample volume requirements or immediate access to the competitive X-ray beamtime. Obtaining a high volume of well-diffracting, sufficient-size crystals while mitigating radiation damage remains a critical bottleneck of serial crystallography. As an alternative, we introduce the plate-reader module adapted for using a 72-well Terasaki plate for biomacromolecule structure determination at a convenience of a home X-ray source. We also present the first ambient temperature lysozyme structure determined at the Turkish light source (Turkish DeLight). The complete dataset was collected in 18.5 min with resolution extending to 2.39 angstrom and 100% completeness. Combined with our previous cryogenic structure (PDB ID: 7Y6A), the ambient temperature structure provides invaluable information about the structural dynamics of the lysozyme. Turkish DeLight provides robust and rapid ambient temperature biomacromolecular structure determination with limited radiation damage.en_US
dc.description.sponsorshipNSF Science and Technology Center [NSF-1231306]; Scientific and Technological Research Council of Tuerkiye (TUEBITAK) [118C476]; TUEBITAK [118C225, 118C270, 121C063, 120Z520]en_US
dc.description.sponsorshipAuthors would like to dedicate this manuscript to the memory of Dr. Albert E. Dahlberg and Dr. Nizar Turker. The authors gratefully acknowledge use of the services and facilities of the Koc University Isbank Infectious Disease Center (KUISCID). H.D. acknowledges support from NSF Science and Technology Center grant NSF-1231306 (Biology with X-ray Lasers, BioXFEL). A.K. acknowledges support from Scientific and Technological Research Council of Tuerkiye (TUEBITAK, 2218-National Postdoctoral Research Fellowship Program under project number 118C476). B.V.K. is funded by TUEBITAK 2232 International Outstanding Researchers Program (Project No: 118C225). This publication has been produced benefiting from the 2232 International Fellowship for Outstanding Researchers Program, 2236 CoCirculation2 program and the 1001 Scientific and Technological Research Projects Funding Program of the TUEBITAK (Project Nos. 118C270, 121C063 and 120Z520). However, the entire responsibility of the publication belongs to the authors of the publication. The financial support received from TUEBITAK does not mean that the content of the publication is approved in a scientific sense by TUEBITAK. Coordinates of the lysozyme structure have been deposited in the Protein Data Bank under accession code 8H3W.en_US
dc.identifier.doi10.1038/s41598-023-33989-0
dc.identifier.issn2045-2322
dc.identifier.issue1en_US
dc.identifier.pmid37208392en_US
dc.identifier.scopus2-s2.0-85159710572en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-023-33989-0
dc.identifier.urihttps://hdl.handle.net/11454/102181
dc.identifier.volume13en_US
dc.identifier.wosWOS:001001529400055en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNature Portfolioen_US
dc.relation.ispartofScientific Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectProtein Crystallographyen_US
dc.subjectSerial Crystallographyen_US
dc.subjectRadiation-Damageen_US
dc.titleRapid and efficient ambient temperature X-ray crystal structure determination at Turkish Light Sourceen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu