Synthesis, characterization and toxicity assessment of a new polymeric nanoparticle, L-glutamic acid-g-p(HEMA)

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dc.contributor.authorBakan, Buket
dc.contributor.authorGulcemal, Suleyman
dc.contributor.authorAkgol, Sinan
dc.contributor.authorHoet, Peter H. M.
dc.contributor.authorYavasoglu, N. Ulku Karabay
dc.date.accessioned2020-12-01T12:04:59Z
dc.date.available2020-12-01T12:04:59Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractThe toxic effects of poly(HEMA)-based polymeric nanoparticles must be analyzed before their biomedical applications as drug delivery systems. the aim of the study was to characterize and evaluate the toxicity for its biocompatibility of a newly synthesized L-glutamic acid-g-p(HEMA) polymeric nanoparticle the nanoparticle was synthesized with surfactant-free emulsion polymerization and grafting techniques. Grafting efficiency was estimated at 58%. the nanoparticle shape was verified as nearly spherical by scanning electron microscopy. Atomic force microscopy images showed a rough surface topography. the nanoparticle had an average size of similar to 194.6 nm on zeta analysis, and the zeta potential value was -18 mV. Fourier transformed infrared spectroscopy revealed spectra from 750 to 4000 cm(-1) and characteristic peaks of stretching bands. the swelling ratio was 46%. With 24-h exposure, p(HEMA) and L-glutamic acid-g-p(HEMA) did not have cytotoxic effects on a human bronchial epithelial cell line (16HBE) and human monocyte cell line by water-soluble tetrazolium salt 1 (WST-1) assay and lactate dehydrogenase assay (LDH). It did not show genotoxic potential by comet assay and did not have mutagenic effects on Salmonella typhimurium TA98, TA100, TA1535 and TA1537 strains by Ames test. the nanoparticle at 160 mu g/ml showed 2% hemolytic activity on erythrocytes. on cell migration assay, the percentage closure difference between exposed and control cells was estimated at 21%. We found no irritation effect on Hen's egg test-chorioallantoic membrane test. We determined that the polymeric nanoparticle L-glutamic acid-g-p(HEMA) was biocompatible and has potential for use in a drug delivery system.en_US
dc.description.sponsorshipEge University, Scientific Research ProjectEge University [2015/FEN/010]; TUBITAK 2214-A - PhD Abroad Research Scholarship Program, TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThe study was supported by Ege University, Scientific Research Project (Project number is 2015/FEN/010) and TUBITAK 2214-A - PhD Abroad Research Scholarship Program, Turkey.en_US
dc.identifier.doi10.1016/j.cbi.2019.108870en_US
dc.identifier.issn0009-2797
dc.identifier.issn1872-7786
dc.identifier.pmid31669216en_US
dc.identifier.scopus2-s2.0-85078085926en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.cbi.2019.108870
dc.identifier.urihttps://hdl.handle.net/11454/62833
dc.identifier.volume315en_US
dc.identifier.wosWOS:000514572800010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofChemico-Biological Interactionsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectL-glutamic acid-g-p(HEMA)en_US
dc.subjectPolymeric nanoparticleen_US
dc.subjectCytotoxicityen_US
dc.subjectGenotoxicityen_US
dc.subjectHemolysisen_US
dc.titleSynthesis, characterization and toxicity assessment of a new polymeric nanoparticle, L-glutamic acid-g-p(HEMA)en_US
dc.typeArticleen_US

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