Microbial ribonucleases (RNases): production and application potential

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dc.contributor.authorHames, E. Esin
dc.contributor.authorDemir, Tugce
dc.date.accessioned2019-10-27T22:59:14Z
dc.date.available2019-10-27T22:59:14Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractRibonuclease (RNase) is hydrolytic enzyme that catalyzes the cleavage of phosphodiester bonds in RNA. RNases play an important role in the metabolism of cellular RNAs, such as mRNA and rRNA or tRNA maturation. Besides their cellular roles, RNases possess biological activity, cell stimulating properties, cytotoxicity and genotoxicity. Cytotoxic effect of particular microbial RNases was comparable to that of animal derived counterparts. In this respect, microbial RNases have a therapeutic potential as anti-tumor drugs. The significant development of DNA vaccines and the progress of gene therapy trials increased the need for RNases in downstream processes. In addition, RNases are used in different fields, such as food industry for single cell protein preparations, and in some molecular biological studies for the synthesis of specific nucleotides, identifying RNA metabolism and the relationship between protein structure and function. In some cases, the use of bovine or other animal-derived RNases have increased the difficulties due to the safety and regulatory issues. Microbial RNases have promising potential mainly for pharmaceutical purposes as well as downstream processing. Therefore, an effort has been given to determination of optimum fermentation conditions to maximize RNase production from different bacterial and fungal producers. Also immobilization or strain development experiments have been carried out.en_US
dc.identifier.doi10.1007/s11274-015-1945-8en_US
dc.identifier.endpage1862en_US
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue12en_US
dc.identifier.pmid26433394en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1853en_US
dc.identifier.urihttps://doi.org/10.1007/s11274-015-1945-8
dc.identifier.urihttps://hdl.handle.net/11454/51754
dc.identifier.volume31en_US
dc.identifier.wosWOS:000363725700004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWorld Journal of Microbiology & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRibonuclease (RNase)en_US
dc.subjectMicrobial RNaseen_US
dc.subjectProductionen_US
dc.subjectOptimizationen_US
dc.subjectAntitumoren_US
dc.subjectDownstream processen_US
dc.titleMicrobial ribonucleases (RNases): production and application potentialen_US
dc.typeReview Articleen_US

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