Alterations in the expression of the apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/Ref-1) and DNA damage in the caudal region of acute and chronic spinal cord injured rats treated by embryonic neural stem cells

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dc.contributor.authorDagci T.
dc.contributor.authorArmagan G.
dc.contributor.authorKonyalioglu S.
dc.contributor.authorYalcin A.
dc.date.accessioned2019-10-26T22:57:25Z
dc.date.available2019-10-26T22:57:25Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractThe oxidative mechanisms of injury-induced damage of neurons within the spinal cord are not very well understood. We used a model of T8-T9 spinal cord injury (SCI) in the rat to induce neuronal degeneration. In this spinal cord injury model, unilateral avulsion of the spinal cord causes oxidative stress of neurons. We tested the hypothesis that apurinic/apyrimidinic endonuclease (or redox effector factor-1, APE/Ref-1) regulates this neuronal oxidation mechanism in the spinal cord region caudal to the lesion, and that DNA damage is an early upstream signal. The embryonic neural stem cell therapy significantly decreased DNA damage levels in both study groups - acutely (followed up to 7 days after SCI), and chronically (followed up to 28 days after SCI) injured animals. Meanwhile, mRNA levels of APE/Ref-1 significantly increased after embryonic neural stem cell therapy in acutely and chronically injured animals when compared to acute and chronic sham groups. Our data has demonstrated that an increase of APE/Ref-1 mRNA levels in the caudal region of spinal cord strongly correlated with DNA damage after traumatic spinal cord injury. We suggest that DNA damage can be observed both in lesional and caudal regions of the acutely and chronically injured groups, but DNA damage is reduced with embryonic neural stem cell therapy. © 2009 Institute of Physiology, Czech Academy of Sciences.en_US
dc.identifier.endpage434en_US
dc.identifier.issn0862-8408
dc.identifier.issue3en_US
dc.identifier.pmid18637713en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage427en_US
dc.identifier.urihttps://hdl.handle.net/11454/20471
dc.identifier.volume58en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofPhysiological Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAPE proteinen_US
dc.subjectDNA damageen_US
dc.subjectRef-1 proteinen_US
dc.subjectSpinal cord injuryen_US
dc.subjectStem cell transplantationen_US
dc.titleAlterations in the expression of the apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/Ref-1) and DNA damage in the caudal region of acute and chronic spinal cord injured rats treated by embryonic neural stem cellsen_US
dc.typeArticleen_US

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