Application of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albumin

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dc.contributor.authorAvcibasi, Nesibe
dc.contributor.authorUygun, Murat
dc.contributor.authorCorman, M. Emin
dc.contributor.authorAkgol, Sinan
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-27T21:28:58Z
dc.date.available2019-10-27T21:28:58Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractSupermacroporous poly{2-hydroxyethyl methacrylate-co-[N,N-bis(2,6-diisopropylphenyl)-perylene-3,4,9,10-tetracarboxylic diimide]} [poly(HEMA-co-DIPPER)] monolithic cryogel column was prepared by radical cryocopolymerization of HEMA with DIPPER as functional comonomer and N,N'-methylene-bisacrylamide (MBAAm) as crosslinker directly in a plastic syringe for adsorption of albumin. The monolithic cryogel contained a continuous polymeric matrix having interconnected pores of 10-50 mu m size. Poly(HEMA-co-DIPPER) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly (HEMA-co-DIPPER) cryogel was 14.7 g H2O/g dry cryogel. Poly(HEMA-co-DIPPER) cryogel was used in the adsorption/desorption of albumin from aqueous solutions. The nonspecific adsorption of albumin onto plain poly(HEMA) cryogel was very low (3.36 g/g polymer). The maximum amount of albumin adsorption from aqueous solution in acetate buffer was 40.9 mg/g polymer at pH 5.0. It was observed that albumin could be repeatedly adsorbed and desorbed with the poly(HEMA-co-DIPPER) cryogel without significant loss of adsorption capacity.en_US
dc.identifier.doi10.1007/s12010-010-8997-xen_US
dc.identifier.endpage2243en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue8en_US
dc.identifier.pmid20521122en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2232en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-010-8997-x
dc.identifier.urihttps://hdl.handle.net/11454/45239
dc.identifier.volume162en_US
dc.identifier.wosWOS:000284295000011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryogelen_US
dc.subjectAffinity separationen_US
dc.subjectAlbuminen_US
dc.subjectHEMAen_US
dc.titleApplication of Supermacroporous Monolithic Hydrophobic Cryogel in Capturing of Albuminen_US
dc.typeArticleen_US

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