Doxycycline down-regulates matrix metalloproteinase expression and inhibits NF-?b signalling in LPS-induced PC3 cells

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dc.contributor.authorOgut D.
dc.contributor.authorReel B.
dc.contributor.authorKorkmaz C.G.
dc.contributor.authorArun M.Z.
dc.contributor.authorMicili S.C.
dc.contributor.authorErgur B.U.
dc.date.accessioned2019-10-27T08:20:50Z
dc.date.available2019-10-27T08:20:50Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractIntroduction. Matrix metalloproteinase enzymes (MMPs) play important role in inflammation, malignant cell proliferation, invasion and angiogenesis by mediating extracellular matrix degradation. Doxycycline, a synthetic tetracycline, behaves as a MMP inhibitor at a subantimicrobial dose and inhibits tumor cell proliferation, invasion and angiogenesis. The aberrant activity of nuclear factor kappa B (NF-?B) causes activation of MMPs and thereby proliferation and invasion of cancer cells. The aim of this study was to investigate the effects of doxycycline on the expression of MMPs in lipopolysaccharide (LPS)-induced PC3 human prostate cancer cells and the possible role of NF-?B signaling. Material and methods. PC3 cells were incubated with LPS (0.5 µg/mL) for 24 h in the presence or absence of doxycycline (5 µg/mL). The effects of LPS and doxycycline on the expressions of MMP-2, MMP-8, MMP-9, MMP-10, NF-?B/p65, I?B-?, p-I?B-?, IKK-ß were examined by Western blotting and immunohistochemistry in PC3 cells. Furthermore, relative proteinase activities of MMP-2 and MMP-9 were determined by gelatin zymography. Results. LPS increased expression and activity of MMP-9 and expression of MMP-8, MMP-10, NF-?B/p65, p-I?B-?, IKK-ß and doxycycline down-regulated its effects with the exception of MMP-10 expression. The expression of MMP-2 and I?B-a was affected by neither LPS nor doxycycline. Conclusions. Our findings indicate that doxycycline inhibits the expression of various MMPs and NF-?B signaling may play a role in the regulation of MMPs expression in LPS-induced PC3 human prostate cancer cells. ©Polish Society for Histochemistry and Cytochemistry.en_US
dc.identifier.doi10.5603/FHC.a2016.0022en_US
dc.identifier.endpage180en_US
dc.identifier.issn0239-8508
dc.identifier.issue4en_US
dc.identifier.pmid27966209en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://doi.org/10.5603/FHC.a2016.0022
dc.identifier.urihttps://hdl.handle.net/11454/25824
dc.identifier.volume54en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVia Medicaen_US
dc.relation.ispartofFolia Histochemica et Cytobiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDoxycyclineen_US
dc.subjectImmunohistochemistryen_US
dc.subjectMMPsen_US
dc.subjectNF-?Ben_US
dc.subjectPC3 cellsen_US
dc.subjectProstate canceren_US
dc.subjectWestern blottingen_US
dc.subjectZymographyen_US
dc.titleDoxycycline down-regulates matrix metalloproteinase expression and inhibits NF-?b signalling in LPS-induced PC3 cellsen_US
dc.typeArticleen_US

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