Subgingival plaque in periodontal health antagonizes at Toll-like receptor 4 and inhibits E-selectin expression on endothelial cells

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dc.contributor.authorTo T.T.
dc.contributor.authorGümüş P.
dc.contributor.authorNizam N.
dc.contributor.authorBuduneli N.
dc.contributor.authorDarveau R.P.
dc.date.accessioned2019-10-27T08:21:24Z
dc.date.available2019-10-27T08:21:24Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThe ability of the subgingival microbial community to induce an inappropriate inflammatory response ultimately results in the destruction of bone and gingival tissue. In this study, subgingival plaque samples from both healthy and diseased sites in the same individual were obtained from adults with chronic periodontitis and screened for their ability to either activate Toll-like receptor 2 (TLR2) or TLR4 and to antagonize TLR4-specific activation by agonist, Fusobacterium nucleatum LPS. Subgingival plaque from diseased sites strongly activated TLR4, whereas matched plaque samples obtained from healthy sites were significantly more variable, with some samples displaying strong TLR4 antagonism, while others were strong TLR4 agonists when combined with F. nucleatum LPS. Similar results were observed when TLR4 dependent E-selectin expression by endothelial cells was determined. These results are the first to demonstrate TLR4 antagonism from human plaque samples and demonstrate that healthy but not diseased sites display a wide variation in TLR4 agonist and antagonist behavior. The results have identified a novel characteristic of clinically healthy sites and warrant further study on the contribution of TLR4 antagonism in the progression of a healthy periodontal site to a diseased one. © 2015, American Society for Microbiology.en_US
dc.description.sponsorshipNational Institutes of Health, NIH: T90DE021984en_US
dc.description.sponsorshipThis study was funded by National Institute of Dental and Craniofacial Research grants awarded to the University of Washington School of Dentistry and T.T.T. (T90DE21984) and awarded to R.P.D. (R01DE012768). HHS | NIH | National Institute of Dental and Craniofacial Research (NIDCR) provided funding to Richard P. Darveau under grant number R01DE012768. HHS | NIH | National Institute of Dental and Craniofacial Research (NIDCR) provided funding to Thao T. To under grant number T90DE21984. --en_US
dc.identifier.doi10.1128/IAI.00693-15en_US
dc.identifier.endpage126en_US
dc.identifier.issn0019-9567
dc.identifier.issue1en_US
dc.identifier.pmid26483407en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage120en_US
dc.identifier.urihttps://doi.org/10.1128/IAI.00693-15
dc.identifier.urihttps://hdl.handle.net/11454/26009
dc.identifier.volume84en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofInfection and Immunityen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleSubgingival plaque in periodontal health antagonizes at Toll-like receptor 4 and inhibits E-selectin expression on endothelial cellsen_US
dc.typeArticleen_US

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