Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines

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dc.contributor.authorSeda Vatansever H.
dc.contributor.authorSorkun K.
dc.contributor.authorIsmet Deliloglu Gurhan S.
dc.contributor.authorOzdal-Kurt F.
dc.contributor.authorTurkoz E.
dc.contributor.authorGencay O.
dc.contributor.authorSalih B.
dc.date.accessioned2019-10-26T22:29:06Z
dc.date.available2019-10-26T22:29:06Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractPropolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects. The target of this study was to investigate the anti-apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF-7 in culture. Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey. Individual extracts at 0.5, 0.25, 0.125 and 0.063 mg/ml were incubated with MCF-7 cells during 2 days culture. Cell growth and cytotoxicity were measured colorimetrically by MTT assay. Apoptotic cell death was determined by the TUNEL method (terminal deoxynucleotidyltransferase-biotin nick end-labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9. The results showed that the PE 5 and 6 extracts at 0.125 mg/ml dilution induced apoptosis in association with increased number of TUNEL positive cells. MTT results showed that cultures exposed to the same extracts and at the same dilution experienced better cell growth compared to those cultures exposed to the other extracts. Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9. Caspase 6 labelling was especially strong in PE 5 and PE 6. We conclude that propolis may have anti-tumour effects by increasing apoptosis through the caspase pathway. Such propolis extracts may be important economically and allow development of a relatively inexpensive cancer treatment. © 2009 Elsevier GmbH.en_US
dc.identifier.doi10.1016/j.acthis.2009.06.001en_US
dc.identifier.endpage556en_US
dc.identifier.issn0065-1281
dc.identifier.issue6en_US
dc.identifier.pmid19733388en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage546en_US
dc.identifier.urihttps://doi.org/10.1016/j.acthis.2009.06.001
dc.identifier.urihttps://hdl.handle.net/11454/19663
dc.identifier.volume112en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofActa Histochemicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectBreast carcinomaen_US
dc.subjectCaspaseen_US
dc.subjectMCF-7 cellen_US
dc.subjectPropolisen_US
dc.titlePropolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell linesen_US
dc.typeArticleen_US

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