Development of a chemometric method in urine sample for newly designed hallucinating psychoactive substances: 5-MeO-MiPT

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dc.contributor.authorEmen, Ezgi
dc.contributor.authorAnnette Akgür, Serap
dc.contributor.authorAslan, Rukiye
dc.contributor.authorAydoğdu, Melike
dc.contributor.authorErtaş, Hasan
dc.date.accessioned2024-08-25T18:38:16Z
dc.date.available2024-08-25T18:38:16Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractAim: In this study, a method was developed for analysis and chemometric optimization for 5-methoxy N-methyl-N-isopropyltryptamine (5-MeO-MiPT). Materials and Methods: Our study was carried out in Ege University Institute on Drug Abuse, Toxicology and Pharmaceutical Science, Addiction Toxicology Laboratory. Analysis and optimization of the effects of hydrolysis and solid phase extraction processes during the analysis of 5-MeO-MiPT by Gas Chromatography-Mass Spectrometry (GC-MS). For chemometric screenings design, Plackett-Burman was used. The most effective three factors were determined, and a central composite design was applied, and results were evaluated with surface response methodology. The method was validated for selectivity, linearity, the limit of detection, the limit of quantitation, accuracy, intra-day and inter-day repeatability, stability and carry over. Results: In the chemometric method, the most effective parameters were found sample volume, hydrolysis temperature, and elution volume for 5-MeO-MiPT in urine analysis. Optimum values for these parameters were calculated by surface response methodology and the results were determined 1ml urine volume, 30°C hydrolysis temperature, 3,5 ml elution volume, respectively. The optimized method was validated for selectivity, linearity (25-500 ng/mL), limit of detection (5 ng/mL), limit of quantitation (18 ng/mL), accuracy (72-101%), intra-day and inter-day precisions were measured, respectively (4,43% RSD),(4,27% CV), stability and carry over parameters. Conclusion: With a chemometric approach, quick, practical and accurate method for the detection of 5-MeO-MiPT has been developed with GC-MS. Working of 5-MeO-MiPT without derivatization in GC MS analysis has shortened the pre-preparation time and is a pioneer for other analogs. It provides an effective method in the analysis of substances such as synthetic analogues from tryptamines which are added every day, with the use of such classical equipment and new methods.en_US
dc.identifier.endpage363en_US
dc.identifier.issn1016-9113
dc.identifier.issn2147-6500
dc.identifier.issue3en_US
dc.identifier.startpage355en_US
dc.identifier.trdizinid1196597en_US
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1196597
dc.identifier.urihttps://hdl.handle.net/11454/100931
dc.identifier.volume62en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofEge Tıp Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.titleDevelopment of a chemometric method in urine sample for newly designed hallucinating psychoactive substances: 5-MeO-MiPTen_US
dc.typeArticleen_US

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