The Effects of Different Pressure Pneumoperitoneum Models Created By Standard or Heated-Humidified CO2 Insufflation on Ovary and Peritoneum: an Experimental Study in Rats

Basit Öğe Kaydı
dc.authoridGunusen, ilkben/0000-0002-8719-0358
dc.authoridKUŞÇU, Gökçe Ceren/0000-0003-1750-8209
dc.authoridKaraman, Semra/0000-0003-0933-3090
dc.authoridCELIK, Kubra/0000-0002-0161-6179
dc.authoridsargin, asuman/0000-0003-1606-1548
dc.authoridTaskiran, Dilek/0000-0002-4505-0939
dc.authorscopusid8683168800
dc.authorscopusid35955775000
dc.authorscopusid57208215503
dc.authorscopusid36919303500
dc.authorscopusid6603891738
dc.authorscopusid57208213974
dc.authorscopusid57377969200
dc.authorwosidGunusen, ilkben/AFV-5158-2022
dc.authorwosidGUREL, ÇEVİK/AAA-1277-2021
dc.authorwosidsargin, asuman/T-7242-2018
dc.authorwosidKUŞÇU, Gökçe Ceren/B-6557-2016
dc.contributor.authorGunusen, Ilkben
dc.contributor.authorAkdemir, Ali
dc.contributor.authorGurel, Cevik
dc.contributor.authorSargin, Asuman
dc.contributor.authorTaskiran, Dilek
dc.contributor.authorKuscu, Gokce Ceren
dc.contributor.authorCelik, Kubra
dc.date.accessioned2023-01-12T19:54:18Z
dc.date.available2023-01-12T19:54:18Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractThere is still controversy over whether structural and morphological changes can be observed in tissues depending on the carbon dioxide (CO2) nature or the applied intra-abdominal pressures (IAP). This study aimed to investigate the effects of different pressure or CO2 nature used for pneumoperitoneum in gynecological laparoscopic surgery on inflammation, DNA damage, oxidative stress, and histopathological changes in ovarian and peritoneal tissue. For this purpose, forty female rats were randomly divided into 6 groups and different pneumoperitoneum models were created in these groups. Rats in group other than control and sham groups received standard (CD) or heated-humidified CO2 (HH) insulations at low (4 mmHg) or high pressure (8 mmHg). The ovary and peritoneum sections were evaluated microscopically for apoptotic index (API) and API scoring was calculated. Tissue and plasma interleukin-6 (IL-6), tumor necrotizing factor-alpha (TNF-alpha), anti-Mullerian hormone (AMH) and 8-hydroxydeoxyguanosine (8-OHdG) levels were analyzed with enzyme-linked immunosorbent assay (ELISA). The most severe changes occurred in the 8CD group microscopically, while the least severe changes occurred in the 4HH group. All histopathological parameters except for ovarian apoptotic index and peritoneal PCNA at low pressure were higher in the CD group. TNF-alpha and 8-OHdG levels were higher in the CD group at both low and high pressures. Standard CO2 caused more prominent histopathological changes at high pressures and systemic inflammation in both pressure groups. The least change between the experimental study groups in terms of histopathological and biochemical was observed in the low-pressure heated-humidified group.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [TGA-2019-20808]en_US
dc.description.sponsorshipThis study was supported by the Ege University Scientific Research Projects Coordination Unit (grant number: TGA-2019-20808 (to Ilkben Gunusen)).en_US
dc.identifier.doi10.1007/s43032-022-00878-2
dc.identifier.endpage1208en_US
dc.identifier.issn1933-7191
dc.identifier.issn1933-7205
dc.identifier.issue4en_US
dc.identifier.pmid35157261en_US
dc.identifier.scopus2-s2.0-85124456080en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1197en_US
dc.identifier.urihttps://doi.org/10.1007/s43032-022-00878-2
dc.identifier.urihttps://hdl.handle.net/11454/76360
dc.identifier.volume29en_US
dc.identifier.wosWOS:000754934200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofReproductive Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaparoscopyen_US
dc.subjectCO2 natureen_US
dc.subjectIntra-abdominal pressureen_US
dc.subjectApoptosisen_US
dc.subjectDNA damageen_US
dc.subjectInflammationen_US
dc.subjectCarbon-Dioxide Pneumoperitoneumen_US
dc.subjectIschemia-Reperfusion Injuryen_US
dc.subjectOxidative Stressen_US
dc.subjectIntraperitoneal Pressureen_US
dc.subjectBody-Temperatureen_US
dc.subjectAntioxidanten_US
dc.subjectImpacten_US
dc.subjectApoptosisen_US
dc.subjectSurgeryen_US
dc.subjectOocytesen_US
dc.titleThe Effects of Different Pressure Pneumoperitoneum Models Created By Standard or Heated-Humidified CO2 Insufflation on Ovary and Peritoneum: an Experimental Study in Ratsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu