Gold nanoparticle probes: Design and in vitro applications in cancer cell culture

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dc.contributor.authorUnak, Gulcan
dc.contributor.authorOzkaya, Feriha
dc.contributor.authorMedine, E. Ilker
dc.contributor.authorKozgus, Ozge
dc.contributor.authorSakarya, Serhan
dc.contributor.authorBekis, Recep
dc.contributor.authorUnak, Perihan
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T21:33:41Z
dc.date.available2019-10-27T21:33:41Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractA new architecture has been designed by the conjugation of [(18)F]2-fluoro-2-deoxy-D-glucose ((18)F-FDG), gold nanoparticles (AuNPs), and anti-metadherin (Anti-MTDH) antibody which is specific to the metadherin (MTDH) over-expressed on the surface of breast cancer cells. Mannose triflate molecule is used as a precursor for synthesis of (18)F-FDG by nucleophilic fluorination. For the conjugation of (18)F-FDG and AuNPs, cysteamine was first bound to mannose triflate (Man-CA) before synthesizing of (18)F-FDG which has cysteamine sides ((18)FDG-CA). Then, (18)FDG-CA was reacted with HAuCl(4) to obtain AuNPs and with NaBH(4) for reduction of AuNPs. At the end of this procedure. AuNPs were conjugated to (18)F-FDG via disulphide bonds ((18)FDG-AuNP). For the conjugation of Anti-MTDH, 1,1'-carbonyl diimidazol (CDI) was bound to the (18)FDG-AuNP, and Anti-MTDH was conjugated via CDI ((18)FDG-AuNP-Anti-MTDH). This procedure was also performed by using Na(19)F to obtain non-radioactive conjugates ((19)FDG-AuNP-Anti-MTDH). Scanning electron microscopy (SEM) images demonstrated that synthesized particles were in nano sizes. (18)FDG-AuNP-Anti-MTDH conjugate was characterized and used as a model probe containing both radioactive and optical labels together as well as the biological target. The (18)FDG-AuNP-Anti-MTDH conjugate was applied to MCF7 breast cancer cell line and apoptotic cell ratio was found to be increasing from 2% to 20% following the treatment. Hence, these results have promised an important application potential of this conjugate in cancer research. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipEge UniversityEge University [2009 FEN 007]en_US
dc.description.sponsorshipThis work was supported by Ege University Research Fund (Project No. 2009 FEN 007). Prof. K.-H. Feller from Jena University is acknowledged for the mass analyses. Prof. Y. Karasulu from Ege University is also acknowledged for her valuable helps during the zeta potential measurements.en_US
dc.identifier.doi10.1016/j.colsurfb.2011.10.027en_US
dc.identifier.endpage226en_US
dc.identifier.issn0927-7765
dc.identifier.pmid22070896en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage217en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2011.10.027
dc.identifier.urihttps://hdl.handle.net/11454/45306
dc.identifier.volume90en_US
dc.identifier.wosWOS:000298518600033en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGold nanoparticles (AuNPs)en_US
dc.subject(18)F-FDGen_US
dc.subjectAnti-metadherin (Anti-MTDH)en_US
dc.subjectTumor imagingen_US
dc.subjectCancer diagnosisen_US
dc.subjectMCF7en_US
dc.titleGold nanoparticle probes: Design and in vitro applications in cancer cell cultureen_US
dc.typeArticleen_US

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