Molecular characterization of hepatitis A virus isolated from acute infections in Turkey

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dc.contributor.authorDinc, Bedia
dc.contributor.authorKoyuncu, Duygu
dc.contributor.authorKaratayli, Senem Ceren
dc.contributor.authorBerk, Elife
dc.contributor.authorKaratayli, Ersin
dc.contributor.authorParlak, Mehmet
dc.contributor.authorCelik, Inci
dc.contributor.authorAkguc, Miray
dc.contributor.authorSertoz, Ruchan
dc.contributor.authorBerktas, Mustafa
dc.contributor.authorBozdayi, Gulendam
dc.contributor.authorBozdayi, A. Mithat
dc.date.accessioned2019-10-27T21:55:55Z
dc.date.available2019-10-27T21:55:55Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground/aims: Hepatitis A virus is a global public health problem, especially in developing countries, and the most common cause of hepatitis in childhood. Hepatitis A virus is a single- stranded positive RNA virus subdivided to 6 genotypes (3 human, 3 simian). The aim of this study was to determine the prevalent genotype in Turkey using sera of acute hepatitis A virus-infected patients from different geographical regions of the country. Materials and Methods: Sera of 137 patients with acute hepatitis A virus from different geographical regions were collected for phylogenetic analysis. The VP1-2A region of the hepatitis A virus genome was amplified by real-time-polymerase chain reaction in 76 patients where possible. Amplified polymerase chain reaction fragments were sequenced, and phylogenetic analysis was done together with other reference hepatitis A virus sequences obtained from GenBank database. Results: Sequencing and phylogenetic analysis of the VP1-2A junction of hepatitis A virus showed that the most prevalent genotype in Turkey is IB (100%). Comparison of Turkish isolates and reference sequences of genotype IB showed a similarity of 94.9%. The same comparison was done between Turkish isolates and reference hepatitis A virus genotype IB and HM175, and it was found that similarity between them ranged from 93.0-95.9%. When Turkish isolates were compared according to Mean Percentage Nucleotide Distance analysis, similarity ranged between 95.3%400%. Conclusions: Phylogenetic analysis pointed out that all Turkish isolates belong to genotype IB. Sequence analysis is a useful tool in revealing hepatitis A outbreaks, and allows us to detect and distinguish the presence of epidemic and small outbreaks.en_US
dc.identifier.doi10.4318/tjg.2012.0450en_US
dc.identifier.endpage719en_US
dc.identifier.issn1300-4948
dc.identifier.issue6en_US
dc.identifier.pmid23794310en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage714en_US
dc.identifier.urihttps://doi.org/10.4318/tjg.2012.0450
dc.identifier.urihttps://hdl.handle.net/11454/48291
dc.identifier.volume23en_US
dc.identifier.wosWOS:000317376400015en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Soc Gastroenterologyen_US
dc.relation.ispartofTurkish Journal of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHepatitis A virusen_US
dc.subjectgenotypingen_US
dc.subjectphylogenetic analysisen_US
dc.titleMolecular characterization of hepatitis A virus isolated from acute infections in Turkeyen_US
dc.typeArticleen_US

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