Application of ß-glucuronidase-immobilised silica gel formulation to microfluidic platform for biotransformation of ß-glucuronides

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dc.contributor.authorMuderrisoglu C.
dc.contributor.authorSargin S.
dc.contributor.authorYesil-Celiktas O.
dc.date.accessioned2019-10-27T08:02:07Z
dc.date.available2019-10-27T08:02:07Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: To improve the efficiency of reactions of ß-glucuronidase (GUS)-assisted glucuronic acid (GluA) removal within a microfluidic system. Results: ß-glucuronidase from Helix pomatia was immobilised and characterised in silica-based sol–gel monoliths. Efficiency of the GUS-doped silica monoliths was tested for hydrolysis of p-Nitrophenyl-ß-d-glucuronide (pNP–GluA) in both ml-scaled medium via batch reactions and microfluidic environment via continuous-flow reactions. In the microfluidic platform, within a duration of 150 min of continuous operation (flow rate: 1 µL/min), the obtained highest pNP yield was almost 50% higher than that of the corresponding batchwise reaction. However, increased flow rates (3, 5, and 10 µL/min) resulted in lower conversion yields compared to 1 µL/min. The microfluidic platform demonstrated continuous hydrolytic activity for 7 days with considerable reaction yields while using a small amount of the enzyme. Conclusion: These results revealed that usage of the microreactors has considerable potential to efficiently obtain bioactive GluA-free aglycons from various plant-derived ß-glucuronides for pharmaceutical applications. © 2018, Springer Science+Business Media B.V., part of Springer Nature.en_US
dc.description.sponsorship113M050en_US
dc.description.sponsorshipAcknowledgements The financial support provided by the Scientific and Technological Research Council of Turkey (TUBITAK, 113M050) is highly appreciated. Special thanks are offered to Dr Barbaros Cetin from the Bilkent University Microfluidics & Lab-on-a-chip Research Group for microfabrication studies, and Dr Bogdan Parakhonskiy from the Ghent University for his valuable comments on the manuscript. --en_US
dc.identifier.doi10.1007/s10529-018-2530-7en_US
dc.identifier.endpage780en_US
dc.identifier.issn0141-5492
dc.identifier.issue5en_US
dc.identifier.pmid29497885en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage773en_US
dc.identifier.urihttps://doi.org/10.1007/s10529-018-2530-7
dc.identifier.urihttps://hdl.handle.net/11454/25127
dc.identifier.volume40en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Netherlandsen_US
dc.relation.ispartofBiotechnology Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme immobilisationen_US
dc.subjectGlucuronide hydrolysisen_US
dc.subjectMicroenzyme reactoren_US
dc.subjectMicrofluidic platformen_US
dc.subjectSol–gelen_US
dc.subjectß-glucuronidaseen_US
dc.titleApplication of ß-glucuronidase-immobilised silica gel formulation to microfluidic platform for biotransformation of ß-glucuronidesen_US
dc.typeArticleen_US

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