Restriction fragment length polymorphism analysis and direct sequencing for determination of HBV genotypes in a Turkish population

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dc.contributor.authorSertoz, Ruchan Yazan
dc.contributor.authorErensoy, Selda
dc.contributor.authorPas, Suzan
dc.contributor.authorOzacar, Tijen
dc.contributor.authorNiesters, Hubert
dc.date.accessioned2019-10-27T19:54:41Z
dc.date.available2019-10-27T19:54:41Z
dc.date.issued2008
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to analyze the restriction fragment length polymorphism and direct sequencing results in genotyping of hepatitis B virus from a Turkish population in a clinical virology laboratory. Serum samples of 54 chronic hepatitis B patients attending the Ege University Hospital were studied. Sequences of partial S gene PCR products were analysed and RFLP was performed. Fifty-three isolates could be identified by direct sequencing as genotype D. One sample needed to be cloned and determined as genotype D. Forty-two isolates were genotyped as D with RFLP according to published determinative patterns. Twelve isolates had undefined patterns. Eight of them suggested a mixture of isolates with different patterns and cloning of these samples confirmed the presence of heterogeneous isolates. Four isolates with undefined pattern were determined as genotype D by direct sequencing. All the studied isolates were genotype D. The results of this studied population suggest that RFLP is suitable for HBV genotyping in a routine clinical virology laboratory setting. However sequence analysis and even cloning may be needed to clarify indeterminate results.en_US
dc.identifier.endpage194en_US
dc.identifier.issn1121-7138
dc.identifier.issue2en_US
dc.identifier.pmid18623983en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage189en_US
dc.identifier.urihttps://hdl.handle.net/11454/40550
dc.identifier.volume31en_US
dc.identifier.wosWOS:000256866500005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherEdizioni Int Srlen_US
dc.relation.ispartofNew Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHBVen_US
dc.subjectgenotypingen_US
dc.subjectsequencingen_US
dc.subjectRFLPen_US
dc.titleRestriction fragment length polymorphism analysis and direct sequencing for determination of HBV genotypes in a Turkish populationen_US
dc.typeArticleen_US

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