Sequential secretion of collagenolytic, elastolytic, and keratinolytic proteases in peptide-limited cultures of two Bacillus cereus strains isolated from wool

Basit Öğe Kaydı
dc.contributor.authorAdiguezel, A. C.
dc.contributor.authorBitlisli, B. O.
dc.contributor.authorYasa, I.
dc.contributor.authorEriksen, N. T.
dc.date.accessioned2019-10-27T20:50:44Z
dc.date.available2019-10-27T20:50:44Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractTo characterize the secretion of proteolytic activities against keratin, collagen and elastin in liquid cultures of Bacillus cereus IZ-06b and IZ-06r isolated from wool. Growth of B. cereus IZ-06b and IZ-06r were characterized in batch culture. Both strains needed an organic nitrogen source, were able to grow on wool or peptone as sole carbon and nitrogen sources, and metabolized glucose, maltose and other simple sugars. Proteolytic activities were investigated in batch cultures grown in peptide-restricted, carbon-sufficient medium. Secretion of proteases was induced by peptide limitation while different proteolytic activities appeared sequentially in the growth medium. When the most available components of the peptone were depleted, collagenolytic and elastolytic proteases were produced. These were later replaced by the production of keratinolytic protease. B. cereus can adjust its proteolytic affinity profile in response to the supply of organic nitrogen and sequentially secrete proteases with activities targeted against increasingly inaccessible proteinous substrates as the nutritional availability in the environment deteriorates. Peptide-limited, carbon-sufficient growth media containing no proteinous substrates are well suited for protease production in B. cereus while growth conditions can be adjusted to optimize the proteolytic affinity profiles.en_US
dc.identifier.doi10.1111/j.1365-2672.2009.04200.xen_US
dc.identifier.endpage234en_US
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.issue1en_US
dc.identifier.pmid19302303en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage226en_US
dc.identifier.urihttps://doi.org/10.1111/j.1365-2672.2009.04200.x
dc.identifier.urihttps://hdl.handle.net/11454/43034
dc.identifier.volume107en_US
dc.identifier.wosWOS:000266707700022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus cereusen_US
dc.subjectcollagenaseen_US
dc.subjectelastaseen_US
dc.subjectkeratinaseen_US
dc.subjectnitrogen limitationen_US
dc.titleSequential secretion of collagenolytic, elastolytic, and keratinolytic proteases in peptide-limited cultures of two Bacillus cereus strains isolated from woolen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu