Effects of Melatonin on the Cerebellum of Infant Rat Following Kaolin-Induced Hydrocephalus: a Histochemical and Immunohistochemical Study

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dc.contributor.authorUyanikgil, Yigit
dc.contributor.authorTurgut, Mehmet
dc.contributor.authorBaka, Meral
dc.date.accessioned2019-10-27T11:10:54Z
dc.date.available2019-10-27T11:10:54Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractHydrocephalus is a developmental disorder causing abnormally collected cerebrospinal fluid within the cerebral ventricles. It leads to bigger skulls and many dysfunctions related to the nervous system. Here, we addressed whether exogenous melatonin administration could reverse the clinical features of kaolin-induced hydrocephalus in infantile rats. A controlled double-blinded study was conducted in 2-week-old 45 Wistar albino rats, which were divided into three groups: Group A, the control group, received intracisternal sham injection with solely the needle insertion; group B, the hydrocephalus group, was treated with isotonic NaCl after kaolin injection; and group C, the hydrocephalus + melatonin group, was given i.p. exogenous melatonin at a dose of 0.5 mg/100 g body weight after kaolin injection. Histological and immunohistochemical analyses were performed after the induction of hydrocephalus and melatonin administration. Glial fibrillary acidic protein was stained by immunohistochemical method. TUNEL method was used to define and quantitate apoptosis in the cerebellar tissues. Statistical analysis was performed by nonparametric Kruskal-Wallis H test, and once significance was determined among means, post hoc pairwise comparisons were carried out using Mann-Whitney U test. We found that melatonin administration significantly ameliorated ratio of substantia grisea area/substantia alba area in the cerebellum of infantile rats. Histologically, there was a significant reduction in the number of cerebellar apoptotic cells after the hydrocephalus induced by kaolin (P < 0.05). Our results clearly revealed that the histopathological changes in the cerebellum were reversed by systemic melatonin administration in infantile rats with kaolin-induced hydrocephalus. Nevertheless, further studies are needed to suggest melatonin as a candidate protective drug in children with hydrocephalus.en_US
dc.identifier.doi10.1007/s12311-016-0778-9en_US
dc.identifier.endpage150en_US
dc.identifier.issn1473-4222
dc.identifier.issn1473-4230
dc.identifier.issue1en_US
dc.identifier.pmid27113349en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage142en_US
dc.identifier.urihttps://doi.org/10.1007/s12311-016-0778-9
dc.identifier.urihttps://hdl.handle.net/11454/32443
dc.identifier.volume16en_US
dc.identifier.wosWOS:000393586100016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCerebellumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCerebellumen_US
dc.subjectInfant raten_US
dc.subjectKaolin-induced hydrocephalusen_US
dc.subjectMelatoninen_US
dc.titleEffects of Melatonin on the Cerebellum of Infant Rat Following Kaolin-Induced Hydrocephalus: a Histochemical and Immunohistochemical Studyen_US
dc.typeArticleen_US

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