In vivo effects of malathion on glutathione-S-transferase and acetylcholinesterase activities in various tissues of neonatal rats
Küçük Resim Yok
Tarih
2003
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Glutation-S-transferazın (GST) pestisitleri de içeren bir çok kimyasal bileşiğin detoksifikasyonunda önemli rol oynadığı rapor edilmiştir. Organofosforlu pestisitler asetilkolinesterazın (AChE) kuvvetli inhibitörüdür. Bu çalışma, yenidoğan sıçanların farklı organlarındaki protein seviyesi, GST ve AChE aktiviteleri üzerine organofosforlu bir pestisit olan malationun in vivo etkilerini değerlendirmek için yapılmıştır. Malationla beslenen yetişkin dişi sıçanlarda karaciğer GST aktivitesi .yaklaşık 2 kat artarken, AChE aktivitesi kontrole göre % 20'den daha fazla azalmıştır. Benzer şekilde, yeni doğan sıçanlarda karaciğer ve kalp GST aktiviteleri (0.14 ve 0.035 U/mg protein, sırasıyla) yaklaşık 2 kat artmıştır, fakat beyin GST aktivitesinde (0.074 U/mg protein) bir azalma gözlenmiştir. Karaciğer, beyin, böbrek ve akciğer AChE aktivitelerinde de (0.002, 0.035, 0.046, ve 0.018 U/mg protein, sırasıyla) kontrolle karşılaştırıldığında önemli derecede azalma gözlenmiştir. Protein içeriklerinde önemli bir değişiklik bulunmamaktadır. Sonuçta malationun sıçanlar üzerinde bazı biokimyasal etkilere sahip olduğu saptanmıştır.
Glutathione-S-transferase (GST) has been reported to play an important role in the detoxification of several chemical compounds, including pesticides. Organophosphate pesticides are potent inhibitors of acetylcholinesterase (AChE). The present study was conducted to assess the in vivo effects of the organophosphate pesticide malathion on the activity of GST and AChE and the protein levels of different organs in neonatal rats. GST activity increased approximately two-fold in adult female rats fed malathion, whereas AChE activity decreased less than 20% of the control in the liver. Similarly, liver and heart GST activities (0.14 and 0.035 U/mg protein, respectively) were also increased approximately two-fold in neonatal rats, but in the brain a decrease in GST activity (0.074 U/mg protein) was observed. The acetylcholinesterase activity in the liver, brain, kidney and lung (0.002, 0.035, 0.046, and 0.018 U/mg protein, respectively) seemed to be significantly decreased compared with the control. There was no significant change in protein contents. In conclusion, it is suggested that malathion has some biochemical effects on rats.
Glutathione-S-transferase (GST) has been reported to play an important role in the detoxification of several chemical compounds, including pesticides. Organophosphate pesticides are potent inhibitors of acetylcholinesterase (AChE). The present study was conducted to assess the in vivo effects of the organophosphate pesticide malathion on the activity of GST and AChE and the protein levels of different organs in neonatal rats. GST activity increased approximately two-fold in adult female rats fed malathion, whereas AChE activity decreased less than 20% of the control in the liver. Similarly, liver and heart GST activities (0.14 and 0.035 U/mg protein, respectively) were also increased approximately two-fold in neonatal rats, but in the brain a decrease in GST activity (0.074 U/mg protein) was observed. The acetylcholinesterase activity in the liver, brain, kidney and lung (0.002, 0.035, 0.046, and 0.018 U/mg protein, respectively) seemed to be significantly decreased compared with the control. There was no significant change in protein contents. In conclusion, it is suggested that malathion has some biochemical effects on rats.
Açıklama
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Biyoloji
Kaynak
Turkish Journal of Zoology
WoS Q Değeri
Scopus Q Değeri
Cilt
27
Sayı
3
