Cells-on-nanofibers: Effect of polyethyleneimine on hydrophobicity of poly-epsilon-caprolacton electrospun nanofibers and immobilization of bacteria

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dc.contributor.authorGordegir, Meleknur
dc.contributor.authorOz, Sultan
dc.contributor.authorYezer, Irem
dc.contributor.authorBuhur, Merve
dc.contributor.authorUnal, Betul
dc.contributor.authorDemirkol, Dilek Odaci
dc.date.accessioned2019-10-27T09:43:27Z
dc.date.available2019-10-27T09:43:27Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractAmong other synthetic polymers, poly-E-caprolacton (PCL) nanofibers are one of the most popular ones, especially in tissue engineering application due to its distinct mechanical and chemical properties. However, in some cases, lacking functional group on polymer structure obstructs the covalent modification of the PCL nanofibers for the aim. Herein, polyethyleneimine (PEI) was blended with PCL polymer to provide functional amino groups on the surface of the nanofiber mat. PCL-PEI nanofiber was successfully constructed and preparation parameters were optimized. Scanning electron microscopy (SEM) and contact angle measurements were carried out to characterize the PCL-PEI nanofiber. After characterization, Gluconobacter oxydans was immobilized on the surface by the help of glutaraldehyde chemistry. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements were carried out to prove the success of surface modification. In addition, scanning electron microscopy images were also taken after the immobilization of G. oxydans on PCL-PEI nanofiber mat. For the first time in this study, one microorganism was immobilized onto the electrospun nanofiber mat by covalent modification. In conclusion, PCL-PEI/G. oxydans whole-cell biosensor was tested for sensing of glucose as a model analyte.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [215Z194]; Aliye Uster Foundation; Turkish Academy of Sciences The Young Scientists Award Programme (TUBA-GEBIP2015)en_US
dc.description.sponsorshipAuthors acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK; Project 215Z194) and Aliye Uster Foundation for funding this research. M. Gordegir, S. Oz and M. Buhur thank to TUBITAK 2209 A-Program for the University Students at Undergraduate Level. Dr. D. O Demirkol acknowledges the support from the Turkish Academy of Sciences The Young Scientists Award Programme (TUBA-GEBIP2015).en_US
dc.identifier.doi10.1016/j.enzmictec.2019.03.002en_US
dc.identifier.endpage31en_US
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.pmid31000161en_US
dc.identifier.startpage24en_US
dc.identifier.urihttps://doi.org/10.1016/j.enzmictec.2019.03.002
dc.identifier.urihttps://hdl.handle.net/11454/28870
dc.identifier.volume126en_US
dc.identifier.wosWOS:000466821100004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEnzyme and Microbial Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrospun nanofiberen_US
dc.subjectBiofunctional surfaceen_US
dc.subjectWhole-cell biosensoren_US
dc.subjectG. oxydansen_US
dc.subjectCovalent immobilizationen_US
dc.titleCells-on-nanofibers: Effect of polyethyleneimine on hydrophobicity of poly-epsilon-caprolacton electrospun nanofibers and immobilization of bacteriaen_US
dc.typeArticleen_US

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