Carrier free immobilization and characterization of trypsin

Basit Öğe Kaydı
dc.contributor.authorMenfaatli, Esra
dc.contributor.authorZihnioglu, Figen
dc.date.accessioned2019-10-27T19:53:58Z
dc.date.available2019-10-27T19:53:58Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractPancreatic trypsin was immobilized by cross-linked enzyme aggregates (CLEA) which is a carrier free immobilization method. Ammonium sulfate was chosen for enzyme precipitation which was followed by cross linking of formed aggregates via glutaraldehyde. Concentrations of precipitant and cross linker were respectively optimized as 60% ammonium sulfate and 1% glutaraldehyde. Optimum pH and temperature for CLEA was increased compared to free enzyme. Furthermore, pH, thermal and storage stability were improved. Presence of additives had no effects on enzyme activity. Prepared cross-linked trypsin aggregates are convenient for in situ protein fragmentation and can be used for protein identification.en_US
dc.identifier.doi10.3109/21691401.2013.853178en_US
dc.identifier.endpage144en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue2en_US
dc.identifier.pmid24195581en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage140en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2013.853178
dc.identifier.urihttps://hdl.handle.net/11454/40401
dc.identifier.volume43en_US
dc.identifier.wosWOS:000352084900010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.relation.ispartofArtificial Cells Nanomedicine and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectenzyme immobilizationen_US
dc.subjectcross-linked enzyme aggregatesen_US
dc.subjecttrypsinen_US
dc.titleCarrier free immobilization and characterization of trypsinen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu