New partner proteins containing novel internal recognition motif for human glutaminase interacting protein (hGIP)

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dc.contributor.authorZencir, Sevil
dc.contributor.authorBanerjee, Monimoy
dc.contributor.authorDobson, Melanie J.
dc.contributor.authorAyaydin, Ferhan
dc.contributor.authorFodor, Elfrieda Ayaydin
dc.contributor.authorTopcu, Zeki
dc.contributor.authorMohanty, Smita
dc.date.accessioned2019-10-27T21:52:51Z
dc.date.available2019-10-27T21:52:51Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractRegulation of gene expression in cells is mediated by protein-protein, DNA-protein and receptor-ligand interactions. PDZ (PSD-95/Discs-large/ZO-1) domains are protein-protein interaction modules. PDZ-containing proteins function in the organization of multi-protein complexes controlling spatial and temporal fidelity of intracellular signaling pathways. In general, PDZ proteins possess multiple domains facilitating distinct interactions. The human glutaminase interacting protein (hGIP) is an unusual PDZ protein comprising entirely of a single PDZ domain and plays pivotal roles in many cellular processes through its interaction with the C-terminus of partner proteins. Here, we report the identification by yeast two-hybrid screening of two new hGIP-interacting partners, DTX1 and STAU1. Both proteins lack the typical C-terminal PDZ recognition motif but contain a novel internal hGIP recognition motif recently identified in a phage display library screen. Fluorescence resonance energy transfer and confocal microscopy analysis confirmed the in vivo association of hGIP with DTX1 and STAU1 in mammalian cells validating the previous discovery of S/T-X-V/L-D as a consensus internal motif for hGIP recognition. Similar to hGIP, DTX1 and STAU1 have been implicated in neuronal function. Identification of these new interacting partners furthers our understanding of GIP-regulated signaling cascades and these interactions may represent potential new drug targets in humans. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipUnited States Department of AgricultureUnited States Department of Agriculture (USDA) [2003-35302-12930, 2011-65503-20030]; National Science FoundationNational Science Foundation (NSF) [IOS-0628064]; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [DK082397]; NSERCNatural Sciences and Engineering Research Council of Canada [155268]; [TUBITAK 108T945]en_US
dc.description.sponsorshipThis research was financially supported by United States Department of Agriculture PECASE award 2003-35302-12930, and AFRI award 2011-65503-20030, National Science Foundation Grant IOS-0628064, and National Institutes of Health Grant DK082397 to Smita Mohanty, NSERC Grant 155268 to Melanie Dobson, and the Grant TUBITAK 108T945 to Zeki Topcu.en_US
dc.identifier.doi10.1016/j.bbrc.2013.01.098en_US
dc.identifier.endpage15en_US
dc.identifier.issn0006-291X
dc.identifier.issue1en_US
dc.identifier.pmid23395680en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage10en_US
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2013.01.098
dc.identifier.urihttps://hdl.handle.net/11454/47755
dc.identifier.volume432en_US
dc.identifier.wosWOS:000316038500003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlutaminase interacting proteinen_US
dc.subjectPDZ domainsen_US
dc.subjectYeast two-hybriden_US
dc.subjectProtein-protein interactionsen_US
dc.subjectFluorescence resonance energy transferen_US
dc.subjectConfocal microscopyen_US
dc.titleNew partner proteins containing novel internal recognition motif for human glutaminase interacting protein (hGIP)en_US
dc.typeArticleen_US

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