Development of ionic liquid modified disposable graphite electrodes for label-free electrochemical detection of DNA hybridization related to Microcystis spp
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI AG
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this present study, ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified pencil graphite electrode (IL-PGEs) was developed for electrochemical monitoring of DNA hybridization related to Microcystis spp. (MYC). The characterization of IL-PGEs was performed using microscopic and electrochemical techniques. DNA hybridization related to MYC was then explored at the surface of IL-PGEs using differential pulse voltammetry (DPV) technique. After the experimental parameters were optimized, the sequence-selective DNA hybridization related to MYC was performed in the case of hybridization between MYC probe and its complementary DNA target, noncomplementary (NC) or mismatched DNA sequence (MM), or and in the presence of mixture of DNA target: NC (1:1) and DNA target: MM (1:1). © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Açıklama
Anahtar Kelimeler
Electrochemical DNA biosensor, Ionic liquid, Microcystis spp, Nucleic acid hybridization, Pencil graphite electrode
Kaynak
Sensors (Switzerland)
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
15
Sayı
9
