Evaluation of the genotype MTBDRplus assay for the diagnosis of tuberculosis and rapid detection of rifampin and isoniazid resistance in clinical specimens
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Tarih
2011
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info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada Genotype MTBDRplus testinin klinik örneklerden tüberküloz tanısı ve hızlı rifampin (RIF) ve izoniyazid (INH) saptanmasıdaki performansının belirlenmesi amaçlanmıştır. Yöntem ve gereç: Aralık 2007 ve 2009 tarihleri arasında Ege Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı Mikobakteriyoloji Laboratuvarı’na rutin inceleme için gönderilen 57 hastaya ait toplam 90 klinik örnek (69 balgam örneği, 11 bronkoskopik aspirat, 5 bronkoalveoler lavaj, 4 derin trakeal aspirat, 1 lenf aspiratı) çalışmaya alındı. Bulgular: Toplam 90 klinik örneğin 80’inde (% 88,9) MTDRplus testiyle geçerli sonuç elde edildi. Direkt bakısı pozitif 82 örneğin 74’ünde (% 90,2) MTDRplus ile geçerli sonuç alınırken, direkt bakısı negatif sekiz örneğin ikisinde geçersiz sonuç alındı. MTBDRplus testiyle ilaç duyarlılık testi arasındaki genel uyumluluk oranı RİF direnci için % 97,5 (78/80) ve INH direncini için % 98,8 (79/80) olarak sapatandı. Sonuç: MTBDRplus klinik örneklerde ve kökenlerde RIF ve INH’a dirençli M. tuberculosis’in tanısında kullanılabilecek yararlı bir araçtır. Buna karşın test sonuçları mutlaka kültür ve ilaç duyarlılık testleriyle doğrulanmalıdır.
Aim: To determine the performance of the Genotype MTBDRplus assay for diagnosis of tuberculosis and rapid detection of rifampin (RIF) and isoniazid (INH) resistance in clinical specimens. Materials and methods: A total of 90 clinical specimens of 57 patients (69 sputum samples, 11 bronchoscopic aspirates, 5 bronchoalveolar lavage, 4 deep tracheal aspirate, 1 lymph aspirate) sent to the Ege University Medical Faculty, Department of Medical Microbiology, Mycobacteriology Laboratory between December 2007 and 2009 during the clinical routine were included in the study. Results: Overall 80 valid results were obtained for 90 clinical specimens (88.9%) with MTBDRplus. While 74 of 82 (90.2%) smear positive specimens gave interpretable results by MTDRplus, 2 of 8 smear negative specimens gave invalid results. Th e overall rates of concordance between the results of the MTBDRplus assay and those of the drug susceptibility testing for the assessment of RIF and INH resistance were 97.5% (78/80) and 98.8% (79/80), respectively. Conclusion: Although the MTBDRplus assay could be a useful tool for rapid identifi cation of RIF- and INH-resistant Mycobacterium tuberculosis in both clinical samples and strains, the test results must always be confi rmed by culture and drug susceptibility testing.
Aim: To determine the performance of the Genotype MTBDRplus assay for diagnosis of tuberculosis and rapid detection of rifampin (RIF) and isoniazid (INH) resistance in clinical specimens. Materials and methods: A total of 90 clinical specimens of 57 patients (69 sputum samples, 11 bronchoscopic aspirates, 5 bronchoalveolar lavage, 4 deep tracheal aspirate, 1 lymph aspirate) sent to the Ege University Medical Faculty, Department of Medical Microbiology, Mycobacteriology Laboratory between December 2007 and 2009 during the clinical routine were included in the study. Results: Overall 80 valid results were obtained for 90 clinical specimens (88.9%) with MTBDRplus. While 74 of 82 (90.2%) smear positive specimens gave interpretable results by MTDRplus, 2 of 8 smear negative specimens gave invalid results. Th e overall rates of concordance between the results of the MTBDRplus assay and those of the drug susceptibility testing for the assessment of RIF and INH resistance were 97.5% (78/80) and 98.8% (79/80), respectively. Conclusion: Although the MTBDRplus assay could be a useful tool for rapid identifi cation of RIF- and INH-resistant Mycobacterium tuberculosis in both clinical samples and strains, the test results must always be confi rmed by culture and drug susceptibility testing.
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Kaynak
Turkish Journal of Medical Sciences
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Cilt
41
Sayı
3
