Effects of Glycogen Synthase Kinase Inhibitor on Glioblastoma Multiforme Cell Line via Apoptosis and Cell Signaling Pathways

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dc.contributor.authorÇamlar, Mahmut
dc.contributor.authorAçıkgöz, Eda
dc.contributor.authorDemir, Kenan
dc.contributor.authorUysal, Ayşegül
dc.contributor.authorÖzer, Füsun
dc.contributor.authorSelçuki, Mehmet
dc.contributor.authorAktuğ, Hüseyin
dc.date.accessioned2020-12-01T12:36:42Z
dc.date.available2020-12-01T12:36:42Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractAIM: To investigate the apoptotic and molecular effects of glycogen synthase kinase-3 (GSK-3) in glioblastoma multiforme (GBM). MATERIAL and METHODS: Human primary glioblastoma cell line (U-87 MG) and the human fetal glial cell line (SVGp12) were used. the cells were exposed to the different doses of GSK inhibitor for 24, 48 and 72 hours. Induction of apoptosis was assessed by DNA fragmentation (TUNEL) assay. EGFR and NF-kB expression was evaluated by immunofluorescence analyses. RESULTS: GSK-3 inhibitor IX induced cytotoxicity and apoptosis in dose-dependent manner in GBM cells. Our results indicated that GSK-3 inhibitor IX induces apoptosis, resulting in a significant decrease in the expression of NF-kB and EGF. CONCLUSION: Inhibition through GSK-3 has been found promising in creating therapeutic management of GBM cells. Proliferation, differentiation, cell cycle regulation, and apoptosis are mechanisms that must be interpreted as a whole. Components associated with EGFR, NF-kB, and apoptosis affect the mechanism solely and collectively. Our collective data suggest that GSK-3 inhibitor IX inhibited cellular proliferation and induced apoptotic events by modulating EGFR and NF-kB expression in GBM cells. GSK-3 inhibition holds promise for the development of new approaches for the therapeutic management of GBM cells.en_US
dc.identifier.doi10.5137/1019-5149.JTN.23987-18.2
dc.identifier.endpage521en_US
dc.identifier.issn1019-5149
dc.identifier.issue4en_US
dc.identifier.startpage513en_US
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.23987-18.2
dc.identifier.urihttps://app.trdizin.gov.tr//makale/TXpVMU9UYzBOQT09
dc.identifier.urihttps://hdl.handle.net/11454/67010
dc.identifier.volume29en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject0-Belirleneceken_US
dc.titleEffects of Glycogen Synthase Kinase Inhibitor on Glioblastoma Multiforme Cell Line via Apoptosis and Cell Signaling Pathwaysen_US
dc.typeArticleen_US

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