Gossypol Interferes with Both Type I and Type II Topoisomerase Activities Without Generating Strand Breaks
dc.contributor.author | Senarisoy, Muge | |
dc.contributor.author | Canturk, Pakize | |
dc.contributor.author | Zencir, Sevil | |
dc.contributor.author | Baran, Yusuf | |
dc.contributor.author | Topcu, Zeki | |
dc.date.accessioned | 2019-10-27T21:52:18Z | |
dc.date.available | 2019-10-27T21:52:18Z | |
dc.date.issued | 2013 | |
dc.department | Ege Üniversitesi | en_US |
dc.description.abstract | A considerable number of agents with chemotherapeutic potentials reported over the past years were shown to interfere with the reactions of DNA topoisomerases, the essential enzymes that regulate conformational changes in DNA topology. Gossypol, a naturally occurring bioactive phytochemical is a chemopreventive agent against various types of cancer cell growth with a reported activity on mammalian topoisomerase II. The compounds targeting topoisomerases vary in their mode of action; class I compounds act by stabilizing covalent topoisomerase-DNA complexes resulting in DNA strand breaks while class II compounds interfere with the catalytic function of topoisomerases without generating strand breaks. In this study, we report Gossypol as the interfering agent with type I topoisomerases as well. We also carried out an extensive set of assays to analyze the type of interference manifested by Gossypol on DNA topoisomerases. Our results strongly suggest that Gossypol is a potential class II inhibitor as it blocked DNA topoisomerase reactions with no consequently formed strand breaks. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG108T548] | en_US |
dc.description.sponsorship | This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) (Grant no. TBAG108T548 to ZT). | en_US |
dc.identifier.doi | 10.1007/s12013-012-9468-5 | en_US |
dc.identifier.endpage | 204 | en_US |
dc.identifier.issn | 1085-9195 | |
dc.identifier.issue | 1 | en_US |
dc.identifier.pmid | 23161103 | en_US |
dc.identifier.scopusquality | N/A | en_US |
dc.identifier.startpage | 199 | en_US |
dc.identifier.uri | https://doi.org/10.1007/s12013-012-9468-5 | |
dc.identifier.uri | https://hdl.handle.net/11454/47580 | |
dc.identifier.volume | 66 | en_US |
dc.identifier.wos | WOS:000317860900023 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.language.iso | en | en_US |
dc.publisher | Humana Press Inc | en_US |
dc.relation.ispartof | Cell Biochemistry and Biophysics | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | DNA topoisomerases | en_US |
dc.subject | Gossypol | en_US |
dc.subject | Anticancer drug research | en_US |
dc.subject | DNA strand breaks | en_US |
dc.title | Gossypol Interferes with Both Type I and Type II Topoisomerase Activities Without Generating Strand Breaks | en_US |
dc.type | Article | en_US |