Neuroprotective Effects of Engineered Polymeric Nasal Microspheres Containing Hydroxypropyl-beta-cyclodextrin on beta-Amyloid (1-42)-Induced Toxicity

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dc.contributor.authorYalcin, Ayfer
dc.contributor.authorSoddu, Elena
dc.contributor.authorBayrakdar, Ezgi Turunc
dc.contributor.authorUyanikgil, Yigit
dc.contributor.authorKanit, Lutfiye
dc.contributor.authorArmagan, Guliz
dc.contributor.authorRassu, Giovanna
dc.contributor.authorGavini, Elisabetta
dc.contributor.authorGiunchedi, Paolo
dc.date.accessioned2019-10-27T23:09:03Z
dc.date.available2019-10-27T23:09:03Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractbeta-Amyloid (A beta) plaques are the key neurotoxic assemblies in Alzheimer disease. It has been suggested that an interaction occurs between membrane cholesterol and A beta aggregation in the brain. Cyclodextrins can remove cholesterol from cell membranes and change receptor function. This study aimed to investigate the effect of hydroxypropyl-beta-cyclodextrin (HP-CD) polymeric microspheres, based on chitosan or sodium alginate, on the levels of lipid peroxidation, reactive oxygen species production, and mitochondrial function in brain synaptosomes. The effect of microspheres on DNA fragmentation, the expression of Bcl-2, Bax, and Apex1 mRNAs in rat hippocampus after A beta(1-42) peptide-induced neurotoxicity was also evaluated. Comparison with HP-CD raw material was performed. A beta(1-42) treatment significantly decreased the mitochondrial activity of Apex1 and Bcl-2 mRNAs, induced DNA fragmentation, and increased mRNA levels of Bax. Treatment with HP-CD microspheres against A beta(1-42) significantly reduced DNA fragmentation and increased the Bcl-2/Bax mRNA ratio and mitochondrial function. In addition, HP-CD microspheres used against A beta(1-42) decreased the levels of lipid peroxidation and reactive oxygen species production. These results indicate that nasally administered spraydried HP-CD microspheres are able to provide protection against A beta(1-42)-induced neurotoxicity, due to the suppressed levels of oxidative stress and apoptotic signals in the rat hippocampus. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipEge University Research FoundationEge University [12/ECZ/037]en_US
dc.description.sponsorshipThis study was partially supported by Ege University Research Foundation (Project no: 12/ECZ/037 to A.Y).en_US
dc.identifier.doi10.1016/j.xphs.2016.05.017en_US
dc.identifier.endpage2380en_US
dc.identifier.issn0022-3549
dc.identifier.issn1520-6017
dc.identifier.issue8en_US
dc.identifier.pmid27353207en_US
dc.identifier.startpage2372en_US
dc.identifier.urihttps://doi.org/10.1016/j.xphs.2016.05.017
dc.identifier.urihttps://hdl.handle.net/11454/52499
dc.identifier.volume105en_US
dc.identifier.wosWOS:000381770400016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta-amyloiden_US
dc.subjecthydroxypropyl-beta-cyclodextrinen_US
dc.subjectmicrosphereen_US
dc.subjectspray dryingen_US
dc.subjectnasal administrationen_US
dc.subjectrat hippocampusen_US
dc.subjectoxidative stressen_US
dc.subjectapoptosisen_US
dc.subjectgene expressionen_US
dc.subjectneuroprotective effecten_US
dc.titleNeuroprotective Effects of Engineered Polymeric Nasal Microspheres Containing Hydroxypropyl-beta-cyclodextrin on beta-Amyloid (1-42)-Induced Toxicityen_US
dc.typeArticleen_US

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