A mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey

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dc.contributor.authorKilimcioglu, Ali Ahmet
dc.contributor.authorGirginkardesler, Nogay
dc.contributor.authorKorkmaz, Metin
dc.contributor.authorOzkol, Mine
dc.contributor.authorDuzgun, Fatih
dc.contributor.authorOstan, Ipek
dc.contributor.authorPabuscu, Yuksel
dc.contributor.authorDinc, Gonul
dc.contributor.authorOk, Ulgen Zeki
dc.date.accessioned2019-10-27T22:06:52Z
dc.date.available2019-10-27T22:06:52Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractCystic echinococcosis (CE) is one of the most important zoonotic diseases in a wide geographic area, including Turkey. In the present project, a total of 4275 students from Celal Bayar University, Manisa, Turkey, were screened by ultrasonography (US) and specific antibodies for CE were examined by Western blotting (WB) and ELISA in finger prick blood samples of 2034 of 4275 volunteered students. We aimed to report the apparent prevalence of CE based on different diagnostic procedures and to compare WB and ELISA with US in diagnosis of CE in a mass screening setting. Six new cases were diagnosed as CE by US during the survey. In addition to these cases, three students were also detected to have been previously operated and pathologically confirmed for hepatic CE. US revealed parenchymal changes in these cases in concordance with their operation history; so, the prevalence of CE by US was calculated as 0.21% (9/4275) (95%CI, 0.11-0.39%) among university students in Manisa. Bands were detected at 8, 28, 32, 38, 42, 47, 70 and 90 kDa by WB and the cases were considered to be positive for CE when at least three of the bands were seen together. Apparent prevalence of CE by ELISA and WB were found to be 2.11% (43/2034) (95%CI, 1.57-2.83%) and 0.25% (5/2034) (95%CI, 0.10-0.57%), respectively. Of the six US positive cases, WB was positive in only one case with two cysts in the liver. All of four cases with liver involvement were positive by ELISA. The high prevalence of CE among university students in Manisa indicated that CE is a major health problem in this area of Turkey. Our results supported that WB is rather difficult and not feasible as a mass screening test and may not be effective for confirmation especially in asymptomatic cases. As a result, we recommend US to be used initially in mass screening surveys for CE followed by confirmation by ELISA for suspected cases. Further examination primarily by chest X-ray followed by computed tomography and/or magnetic resonance imaging, if needed, should be recommended for US negative, ELISA and WB positive individuals who may have non-abdominal cysts. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Projects Committee of Celal Bayar UniversityCelal Bayar Universityen_US
dc.description.sponsorshipThe authors would like to thank all the students who participated in the study, the manager and lecturers of the faculties and schools for their support, to Enis Cezayirli for proof-reading the manuscript and particularly Kehriba Kirbas for her excellent technical laboratory assistance. We also thank Scientific Research Projects Committee of Celal Bayar University for financial support.en_US
dc.identifier.doi10.1016/j.actatropica.2013.08.010en_US
dc.identifier.endpage583en_US
dc.identifier.issn0001-706X
dc.identifier.issn1873-6254
dc.identifier.issue3en_US
dc.identifier.pmid23978681en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage578en_US
dc.identifier.urihttps://doi.org/10.1016/j.actatropica.2013.08.010
dc.identifier.urihttps://hdl.handle.net/11454/48901
dc.identifier.volume128en_US
dc.identifier.wosWOS:000327824800020en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofActa Tropicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCystic echinococcosisen_US
dc.subjectEpidemiologyen_US
dc.subjectUltrasonographyen_US
dc.subjectWestern blottingen_US
dc.subjectELISAen_US
dc.subjectMass screeningen_US
dc.titleA mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkeyen_US
dc.typeArticleen_US

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