Therapeutic versus prophylactic plus therapeutic administration of omega-3 fatty acid on endotoxin-induced peridontitis in rats

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dc.contributor.authorVardar S.
dc.contributor.authorBuduneli E.
dc.contributor.authorTürkoglu O.
dc.contributor.authorBerdeli A.H.
dc.contributor.authorBaylas H.
dc.contributor.authorBaşkesen A.
dc.contributor.authorAtilla G.
dc.date.accessioned2019-10-27T00:12:46Z
dc.date.available2019-10-27T00:12:46Z
dc.date.issued2004
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: The aim of the present study was 1) to evaluate the possible effects of therapeutic usage of omega-3 fatty acid on the gingival tissue levels of prostaglandin E2 (PGE2), prostaglandin F2? (PGF2?), platelet activating factor (PAF), and leukotriene B4 (LTB4) in endotoxin-induced periodontitis in rats and 2) to investigate whether prophylactic usage provides any additional benefits to therapeutic doses of omega-3 fatty acid. Methods: Experimental periodontitis was induced by repeated injection of Escherichia coli lipopolysaccharide (LPS). Thirty-six adult male Sprague-Dawley rats were divided into four study groups: 1) saline controls; 2) LPS; 3) therapeutic omega-3 fatty acid (TO3); and 4) prophylactic plus therapeutic omega-3 fatty acid (P + TO3) groups. In TO3 group, omega-3 fatty acid was given for 15 days following induction of experimental periodontitis. In P + TO3 group, omega-3 fatty acid was started 15 days before baseline, and then periodontitis was induced at baseline and omega-3 fatty acid was continued for 15 days after baseline. On day 15 after baseline, all rats were anesthetized and sacrificed. PGE2, PGF2?, and LTB4 levels in gingival tissue samples were analyzed by enzyme immunoassay and PAF levels were analyzed by radioimmonoassay. Data were evaluated statistically by using parametric tests. Results: LPS injection resulted in significant amount of bone loss (P <0.05). Neither therapeutic nor prophylactic plus therapeutic administration of omega-3 fatty acid with the doses and duration of therapy used in the present study was effective in preventing endotoxin-induced alveolar bone loss. TO3 group exhibited significant decreases in the gingival tissue levels of PGE2, PGF2?, LTB4, and PAF compared to the LPS group (P <0.05). PGE2 and PGF2? levels in TO3 group were similar to those of the saline group (P >0.05), while LTB4 and PAF levels were statistically higher than the saline group (P <0.05). Prophylactic plus therapeutic usage of omega-3 fatty acid provided similar levels of all these mediators to those of the saline controls (P >0.05). Conclusions: Therapeutic omega-3 fatty acid significantly reduced the gingival tissue levels of PGE2, PGF2?, LTB4, and PAF in experimental periodontitis. Furthermore, prophylactic usage of omega-3 fatty acid provided additional beneficial effects to the therapeutic administration by decreasing the gingival tissue levels of these mediators to levels of healthy tissue. These findings should be verified by longitudinal clinical trials investigating clinical and biochemical periodontal parameters to better define the possible role of omega-3 fatty acids in periodontal treatment.en_US
dc.identifier.doi10.1902/jop.2004.75.12.1640en_US
dc.identifier.endpage1646en_US
dc.identifier.issn0022-3492
dc.identifier.issue12en_US
dc.identifier.pmid15732866en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1640en_US
dc.identifier.urihttps://doi.org/10.1902/jop.2004.75.12.1640
dc.identifier.urihttps://hdl.handle.net/11454/22376
dc.identifier.volume75en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlveolar bone lossen_US
dc.subjectAnimal studiesen_US
dc.subjectFatty acids, omega-3/therapeutic useen_US
dc.subjectGingiva/physiologyen_US
dc.subjectLeukotriene B4en_US
dc.subjectProstaglandin E2en_US
dc.subjectProstaglandin F2?en_US
dc.titleTherapeutic versus prophylactic plus therapeutic administration of omega-3 fatty acid on endotoxin-induced peridontitis in ratsen_US
dc.typeArticleen_US

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