A novel biosensor based on L-homocysteine desulfhydrase enzyme immobilized in eggshell membrane

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dc.contributor.authorAlacam, Sencer
dc.contributor.authorTimur, Suna
dc.contributor.authorTelefoncu, Azmi
dc.date.accessioned2019-10-27T19:59:07Z
dc.date.available2019-10-27T19:59:07Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractA novel biosensor for homocysteine determination has been developed. The biosensor was fabricated with L-homocysteine clesulfhydrase immobilized on the ammonium selective electrode by means of eggshell membrane. The measurement principle is based on determination of ammonia due to the enzymatic reaction in the medium by ammonium selective electrode. The effects of enzyme loading, glutaraldehyde concentration, pH, buffer concentration, temperature, dithiotreitol (DTT) concentration and ionic strength adjustment buffer (ISA) on the biosensor response were investigated in detail. The linear detection range and limit of detection (LOD) for homocysteine were found to be 0.15 - 1.8 mM and 55 mu M, respectively. Finally, the homocysteine biosensor has been applied to plasma samples for determination of total homocysteine contents. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.molcatb.2007.08.005
dc.identifier.endpage60en_US
dc.identifier.issn1381-1177
dc.identifier.issn1381-1177en_US
dc.identifier.issue01.Apren_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2007.08.005
dc.identifier.urihttps://hdl.handle.net/11454/41147
dc.identifier.volume49en_US
dc.identifier.wosWOS:000251158300010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Catalysis B-Enzymaticen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthomocysteine biosensoren_US
dc.subjectL-homocysteine desulfhydraseen_US
dc.subjecteggshell membraneen_US
dc.subjectammonium selective electrodeen_US
dc.titleA novel biosensor based on L-homocysteine desulfhydrase enzyme immobilized in eggshell membraneen_US
dc.typeArticleen_US

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